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Effects of 17β-estradiol on the expression of vascular endothelial growth factor in cultured anoxia-injured human retinal pigment epitheliual cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 5
- DOI:
- No doi available
- Key Word:
- 雌激素类/生理学;色素上皮,眼;血管内皮生长因子类;细胞低氧;细胞培养技术
Abstract： 目的 观察雌激素17β-雌二醇(17β-E2)对实验性缺氧损伤的人视网膜色素上皮(RPE)细胞血管内皮生长因子(VEGF)表达和乳酸脱氢酶(LDH)释放率的影响. 方法 用17β-E2和17βE2拮抗剂三苯氧胺(Tamxifen,TAM)预处理RPE细胞后,氯化钴(CoCl2)制备RPE细胞缺氧损伤模型,分别采用比色法、细胞免疫化学法、逆转录聚合酶链式反应(RT-PCR)法检测定正常对照组、缺氧损伤组、17β-E2预处理组及17β-E2和TAM预处理组LDH释放率、VEGF蛋白表达、VEGF mRNA表达情况. 结果 缺氧损伤组RPE细胞较正常对照组LDH释放率增加,VEGF mRNA及蛋白表达增多(P<0.05).17β-E2预处理组与缺氧损伤组比较,VEGF的表达和LDH释放率明显降低(P<0.05);17β-E2作用被TAM阻断后,VEGF表达和LDH释放率增加,与缺氧损伤组比较差差无统计学意义(P>0.05). 结论VEGF在缺氧损伤的人RPE细胞中表达增强;17β-E2可以抑制缺氧损伤后RPE细胞VEGF的表达,降低其LDH释放率,该作用可以为其拮抗剂TAM所阻断.
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