Abstract： Objective To investigate the infection situation and sub-genopype distribution of human papilloma virus (HPV) in female subjects in Taian area.Methods Exfoliated cells from 675 female subjects were harvested with specific cervical brush and DNA samples were prepared for gene chips analysis of 23 genotypes of HPV.Results Totally 189 samples were HPV infected and the percentage of positive HPV was 28.0％.18 subtypes of HPV were screened.High risk subtypes and low risk subtypes of HPV infection rates were 76.72％(145 cases) and 23.28％ (44 cases),respectively.Data also showed that the primary six high risk subtypes were HPV-16 (21.96％),HPV-52 (9.93％),HPV-66 (6.62％),HPV-33 (6.25％),HPV-58 (5.88％) and HPV-18(4.41％).The two main low risk subtypes of HPV were HPV-43 (16.18％) and HPV-11 (12.13％).129cases (68.25％) were single genotype infection; 42 cases (22.22％) and 18 cases (9.52％) were double genotypes infection and multiple genotypes infection,respectively.Conclusions Among female population in Taian area,the infection rate of HPV is 28.0％ and the primary infection manner is single genotype infection.The primary five high risk sub-geontypes are HPV-16,52,66,33 and 58.HPV infection screening and early-stage treament of female subjects employing gene chip methods,as well as follow-up on high risk sub-genotypes infected and mixed genotypes infected patients,are the key points of cervical cancer prevention.