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Biological evaluation of mice and rats exposed to 2.1 MPa and 4.1 MPa H2-O2
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1009-6906.2006.05.003
- Key Word:
- 氢氧环境;毒理学;生物效应;鼠
Abstract： 目的 对处于高压氢氧环境下的小鼠和大鼠进行生物学毒性鉴定及生物效应评价.方法 将实验鼠在2.1 Mpa和4.1 Mpa氢氧环境下暴露1～10 h,以常压和对应高压氦氧环境作为对照.脑组织过氧化损伤、电生理和脑组织兴奋性毒性指标在高压下动态测定,各器官组织学、细胞应激和过氧化损伤指标在高压暴露前后测定.结果 进行高压实验的动物全部存活.小鼠在10 h的氢氧暴露期间以及返回常压后,脑电(EEG)密度谱并未改变.4个月后电镜下脑、肝未见异常.2.1 Mpa氢氧环境下小鼠脑干听觉诱发电位(BAEP)潜伏时比常压下略有延长;在4.1 Mpa氦氧和氢氧环境下BAEP潜伏时进一步延长,以氢氧环境更为明显.2.1 Mpa氦氧和氢氧环境下,大鼠血浆热应激蛋白(HSP-70)含量和小鼠脑细胞外液丙二醛(MDA)含量均略有升高.在大鼠心、肾、肝、脑中,只有肾脏MDA含量在氢氧暴露后出现异常升高.在2.1 Mpa氢氧与氦氧环境下,小鼠脑细胞外液谷氨酸含量未见差异,大鼠脑组织NO含量未呈现差异.结论 在4.1 Mpa高压氢氧环境中,静水压与H2均有阻碍突触传递的作用,并且二者作用是叠加的.在2.1 Mpa高压氢氧环境中,除了肾脏的过氧化负担增加外,脑细胞兴奋性毒性、皮层电活动、细胞应激和细胞形态学指标均说明1～10 h以内的暴露是安全的.
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