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The expression of Bcl-2 in the brain of Wistar rats after carbon monoxide exposure
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1009-6906.2003.03.006
- Key Word:
- Bcl-2基因;一氧化碳;鼠
Abstract： 目的观察一氧化碳(CO)暴露后大鼠脑中Bcl-2基因的分布.方法用Wistar大鼠制成CO暴露动物模型,随机分组,分别在染毒后第3天、第2天和当天处死,取脑制成切片,用地高辛Bcl-2探针原位杂交,观察Bcl-2的信号分布.结果 Bcl-2 mRNA的表达在海马、大脑皮层和胼胝体比较明显,其中海马的表达信号最强,大脑皮层次之,胼胝体最弱.其他部位呈散在的弱信号.相应区域在各个时间点无明显的差异.对照组未见表达. 结论 CO暴露后,Bcl-2在海马、大脑皮层和胼胝体的表达较为明显;Bcl-2在上述部位的表达可能部分的解释CO中毒迟发性神经损伤的发生.
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