The study of killing and proapoptotic effects of topoisomerase I inhibitor on L1210/CDDP cells
- Author:
- No author available
- Journal Title:
- CHINA CLINICAL PRACTICAL MEDICINE
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.1673-8799.2009.03.003
- Key Word:
- 拓扑替康;L1210/CDDP细胞株;凋亡;Caspase-8;Caspase-3;Topotecan;L1210/CDDP cell line;Apoptosis;Caspase-8;Caspase-3
Abstract： Objective Observation and Study of Killing and Proapoptotic Effects of Topoisomerase Ⅰ (topotecan) inhibitor on L1210/CDDP cells. Methods Analyze Topotecan' s killing activity and inductive effect on L1210/CDDP cell lines and the relationship between the above two and Caspase, using MTF method,Annexin V FITC staining, DNA ladder electrophoresis and cell morphology methods. Results TPT on L1210/CDDP cell line has strong anti-role, and a clear time-dependence. At the same time, the target cells present a sign of apoptosis. Caspase-specific inhibitor can cause a certain effect on apoptosis. Conclusion Topotecan' s killing activity and inductive effect on L1210/CDDP cell lines can result in activation of Caspase-8 and Caspase-3.
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