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Influence of immature dendritic cells loading recombinant adenoviral CTLA4Ig on Th cell proliferation
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- Author:
- No author available
- Journal Title:
- JOURNAL OF MODERN CLINICAL MEDICAL BIOENGINEERING
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1008-634X.2007.02.002
- Key Word:
- 负载;CTLA4Ig;重组腺病毒;树突状细胞
Abstract： 目的 探讨负载CTLA4Ig重组腺病毒的未成熟树突状细胞对大鼠Th细胞增殖的影响.方法 将CTLA4Ig重组腺病毒与Wistar大鼠未成熟树突状细胞于37C共孵育6h后,经尾静脉注射该大鼠作实验组,另外分别设立Wistar大鼠未成熟树突状细胞、CTLA4Ig重组腺病毒、生理盐水经尾静脉注射为对照.1周后,用0.3%戊巴比妥麻醉各组大鼠后抽血检测CTLA4Ig.取脾脏,经流式细胞术分选出Th1、Th2细胞及CD4+T细胞,行混合淋巴细胞培养检测Th细胞的增殖.用免疫组织化学法检测Th1、Th2细胞的比例.结果 实验组血清CTLA4Ig水平(0.654±0.13)显著高于CTLA4Ig重组腺病毒组(0.392±0.10,P＜0.01),树突状细胞组及生理盐水组未检出.实验组Th1细胞的增殖指数(742±161)、Th1/Th2(0.16±0.05)均显著低于各对照组(分别与未成熟树突状细胞组、CTLA4Ig重组腺病毒组、生理盐水组相比,P均＜0.01);而Th2细胞的增殖指数(9162 ±598)显著高于各对照组(P均＜0.01).结论 负载CTLA4Ig重组腺病毒的未成熟树突状细胞可显著抑制大鼠Th1细胞增殖,促进Th2细胞增殖,使Th细胞由Th1向Th2显著偏移,诱导有效的免疫耐受.
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