Abstract: Objective To introduce the methods and techniques of treatment of renal pedicle vessels during retroperitoneal laparoscopic nephrectomy.Methods Retroperitoneal laparoscopic nephrectomies were performed in 580 cases in our hospital from March 2004 to May 2014.Different measures were adopted to seek and manage bilateral renal pedicle vessels.The operative time,intraoperative blood loss,duration of postoperative drainage,and postoperative hospital stay were analyzed retrospectively.Results 12 cases were converted to open surgery due to severe bleeding of renal pedicle vessels.Mean operative time was (100.5 ± 24.8) min,mean intraoperative blood loss was (90.4 ± 26.5) ml,duration of postoperative drainage was (3.6 ± 1.2) d,and mean postoperative hospital stay were (7.4 ±2.8) d.Conclusion Different managing for bilateral renal pedicle vessels will help to decrease operative time and improve the safety of the surgery.