Diversity of Lactobacillus in vagina of vulvovaginal candidiasis
- Author:
- No author available
- Journal Title:
- National Medical Journal of China
- Issue:
- 13
- DOI:
- 10.3760/cma.j.issn.0376-2491.2015.13.012
- Key Word:
- 阴道乳杆菌;16S rRNA基因;Vaginal Lactobacillus species;16S rRNA gene
Abstract： Objective To investigate the Lactobacillus species in the vaginas of vulvovaginal candidiasis and to assess the prevalence of each Lactobacillus species in vulvovaginal candidiasis.Methods 154 vaginal samples were analyzed,92 of which were from fertile healthy women,and 62 of which were from women with vulvovaginal candidiasis;and species-specific PCR showed the prevalence of each Lactobacillus species Species-specific PCR was used to investigate the prevalence of each Lactobacillus species in healthy Chinese women and the women with vulvovaginal candidiasis.Results In women with vulvovaginal candidiasis:L.iners (6.5％),L.cripatus (79.0％),L.gasseri (37.1％),L.jensenii (74.2％),L.acidophilus (16.1％),L.brevis (19.4％),L.plantarum (1.6％),L.johnsonii (51.6％),L.fermentum(8.1％),L.salivarius (9.7％),L.reuter (1.6％),L.paracasei (8.1％),L.delbrueckii (3.2％);More than two different Lactobacillus species coexisted in 98％ of women with vulvovaginal candidiasis,and no anyone species existed in 2％ of them;In fertile women:L.iners(82.6％),L.cripatus (70.7％),L.gasseri(67.4％),L.jensenii(40.2％),L.acidophilus(39.1％),L.brevis(23.9％),L.plantarum(5.4％),L.rhamnosus (1.1％),L.paracasei (1.1％),L.reuter (1.1％) i,L.johnsonii (3.3％),L.fermentum (2.2％),L.salivarius (2.2％);More than two different Lactobacillus species coexisted in 97％ of fertile women,and only one species existed in 3％ of fertile women.Conclusions Species of lactobacillus in women with vulvovaginal candidiasis did not significantly reduced compared with healthy women.Lactobacillus inert may be a marker of the change of vaginal microenvironment;Lactobacillus crispatus is a dominant lactobacillus in the vaginal of fertile healthy women,pregnant women and women with vulvovaginal candidiasis.
- [1]Falsen E,Sjoden B,Ohlen M,etc.Phenotypic and phylogenetic characterization of a novel Lactobacillus species from human sources: description of Lactobacillus iners sp. nov.[J].1999,49(1).
- [2]Anderson BL,Fitzsimmons C,Cu Uvin S,etc.Subtle perturbations of genital microflora alter mucosal immunity among low-risk pregnant women.[J].2011,90(5).
- [3]McClelland RS,Richardson BA,Hassan WM,etc.Improvement of vaginal health for Kenyan women at risk for acquisition of human immunodeficiency virus type 1: results of a randomized trial.[J].2008,197(10).
- [4]J D, Sobel,S, Faro,R W, Force,etc.Vulvovaginal candidiasis: epidemiologic, diagnostic, and therapeutic considerations.[J].1998,178(2).
- [5]S L, Hillier,M A, Krohn,R P, Nugent,etc.Characteristics of three vaginal flora patterns assessed by gram stain among pregnant women. Vaginal Infections and Prematurity Study Group.[J].1992,166(3).
- [6]Marilyn Fukushima,Richard W. Hyman,Ronald W. Davis,etc.Microbes on the human vaginal epithelium[J].2005,102(22).
- [7]Liao,Q.,Daroczy,K.,Yu,L.,etc.Qualitative and semiquantitative analysis of Lactobacillus species in the vaginas of healthy fertile and postmenopausal Chinese women[J].2012,61(5).
- [8]Ventura, M,Turroni, F,Klaenhammer, TR,etc.Genome-scale analyses of health-promoting bacteria: probiogenomics[J].2009,7(1).
- [9]Sobel JD.Vulvovaginal candidosis.[J].2007,369(9577).
- [10]DeGregorio,P.R.,Tomás,M.S.J.,Terraf,M.C.L.,etc.In vitro and in vivo effects of beneficial vaginal lactobacilli on pathogens responsible for urogenital tract infections[J].2014,63(Pt.5).
- [11]Xia Zhou,Brown, Celeste J.,Abdo, Zaid,etc.Differences in the composition of vaginal microbial communities found in healthy Caucasian and black women.[J].2007,1(2).
- [12]R P, Nugent,M A, Krohn,S L, Hillier.Reliability of diagnosing bacterial vaginosis is improved by a standardized method of gram stain interpretation.[J].1991,29(2).
- [13]Scholz,C.,Witkin,S.S.,Friese,K.,etc.Relationship between recurrent vulvovaginal candidosis and immune mediators in vaginal fluid[J].2009,144(1).
- [14]Jakobsson T,Forsum U.Lactobacillus iners: a Marker of Changes in the Vaginal Flora?[J].2007,45(9).
- [15]Ferris MJ,Zozaya-Hinchliffe M,Martin DH,etc.Cultivation-independent analysis of changes in bacterial vaginosis flora following metronidazole treatment.[J].2007,45(3).
- [16]Hans, Verstraelen,Rita, Verhelst,Geert, Claeys,etc.Longitudinal analysis of the vaginal microflora in pregnancy suggests that L. crispatus promotes the stability of the normal vaginal microflora and that L. gasseri and/or L. iners are more conducive to the occurrence of abnormal vaginal microflora.[J].2009,9.