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Construction of hyaluronidase gene mutant of Enterococcus faecium and the function of hyaluronidase gene
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- Author:
- No author available
- Journal Title:
- NATIONAL MEDICAL JOURNAL OF CHINA
- Issue:
- 40
- DOI:
- 10.3760/j.issn:0376-2491.2007.40.013
- Key Word:
- 肠球菌,屎;透明质酸葡糖胺酶;突变;模型,动物
Abstract： 目的 构建屎肠球菌类透明质酸酶基因(hyl)突变株,研究hyl基因的功能.方法 用自杀质粒pTX4577通过体内同源重组,筛选获得hyl基因的突变株,研究体外hyl基因缺失对突变株生长能力的影响.通过小鼠腹膜炎和兔心内膜炎模型研究体内突变株的毒力情况.结果 经同源重组,利用卡那霉素抗性筛选,PCR、脉冲场电泳和Southern印迹进行鉴定获得hyl基因突变株*hyl,突变株在体外的生长能力低于野生株.小鼠腹膜炎模型中,在相同细菌感染浓度(3.7×109 CFU/ml)下,野生株TX2466组小鼠在100 h内的存活率为0,而*hyl组小鼠的存活率为50%,差异有统计学意义(P《0.01).兔心内膜炎模型中,TX2466组主动脉瓣和壁性赘生物的菌落计数分别为(3.04±0.63)×106 CFU/g和(6.87±0.58)× 106 CFU/g,而*hyl组则分别为(0.21±0.06)×106 CFU/g和(0.66±0.05)×106 CFU/g,差异有统计学意义(P《0.01).结论 hyl基因突变株构建成功,hyl基因在屎肠球菌致病中起着重要的作用,可能是屎肠球菌的毒力因子之一.
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