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Cardioprotection of recombinant human erythropoietin pretreatment on ischemia-reperfused hearts and mechanism thereof: experiment with rats
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- Author:
- No author available
- Journal Title:
- NATIONAL MEDICAL JOURNAL OF CHINA
- Issue:
- 35
- DOI:
- 10.3760/j.issn:0376-2491.2007.35.005
- Key Word:
- 缺血预处理,心肌;红细胞生成素;核因子-κB;活化蛋白-1
Abstract： 目的 研究重组人促红细胞生成素(rhEPO)预处理对缺血再灌注(I-R)大鼠心肌的保护作用并谈讨其中可能的机制.方法 雄性Sprague-Dawley大鼠共156只,I-R模型采用阻断左前降支30 min后再通3 h,大鼠于24 h前分别腹腔注射生理盐水或rhEPO.结束时测定心肌梗死面积和血浆心肌型肌酸激酶同功酸(CK-MB)水平,观察心肌组织超微结构改变和中性粒细胞浸润情况;酶联免疫吸附试验分别测定再灌注1 h和2 h时心肌肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的含量;凝胶电迁移方法检测心肌核因子-κB(NF-κB)和活化蛋白-1(AP-1)的表达.结果 I-R前24 h单次注射rhEPO(静脉滴注,5000 U/kg)能显著降低I-R后心肌梗死面积,改善心肌细胞超微结构,削弱血浆CK-MB的水平,减轻心肌组织中中性粒细胞的浸润,降低NF-κB和AP-1的激活以及TNF-α和IL-6的表达.结论 rhEPO预处理有显著的心肌保护作用,该作用可能与抑制NF-κB和AP-1的活化及下调下游促炎细胞因子有关.
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