Study of apoptosis and Fas expression of peritoneal fluid and peripheral blood T lymphocytes in patients with epithelial ovarian cancer and their relationship with CA125
- Author:
- No author available
- Journal Title:
- NATIONAL MEDICAL JOURNAL OF CHINA
- Issue:
- 11
- DOI:
- 10.3760/j:issn:0376-2491.2007.11.004
- Key Word:
- 卵巢肿瘤;T淋巴细胞;细胞凋亡;抗原,CD95;CA-125抗原
Abstract： 目的 了解卵巢肿瘤患者外周血和腹水T淋巴细胞凋亡和Fas(CD95)表达特点及其与CA125的关系.方法 应用流式细胞术测定18例Ⅲ～Ⅳ期卵巢癌患者(Ⅲ～Ⅳ期组),15例Ⅰ～Ⅱ期卵巢癌患者(Ⅰ～Ⅱ期组),Ⅲ～Ⅳ期组患者手术加化疗后(治疗后组),18例良性上皮性卵巢肿瘤患者(良性组),6例库肯勃氏瘤患者(库肯勃氏瘤组)及20例正常体检女性(正常组)的外周血T淋巴细胞凋亡和Fas表达,并测定上述治疗前的卵巢癌患者和库肯勃氏瘤患者的腹水T淋巴细胞以及良性组中10例患者(良性盆腔积液组)的盆腔积液T淋巴细胞的凋亡和Fas表达.同时测定卵巢癌患者外周血CA125的水平.结果 Ⅲ～Ⅳ期组患者的外周血T淋巴细胞凋亡阳性率为5.55(3.57～9.62)%,明显高于正常组、良性组、Ⅰ～Ⅱ期组(P＜0.01)和治疗后组(P＜0.05),Ⅲ～Ⅳ期组患者外周血T淋巴细胞的Fas表达强度为51±10,明显高于正常组(P＜0.05).Ⅰ～Ⅱ期组和Ⅲ～Ⅳ期组患者腹水T淋巴细胞的凋亡阳性率,T淋巴细胞的Fas阳性率,Fas表达强度分别为17.41(7.06～24.56)%,(57±16)%,(55±11)%和34.06(17.03～44.65)%,(66±12)%,(70±24)%,均高于良性盆腔积液组的0.78(0.67～1.44)%,(37±6)%,(43±6)%(P＜0.01).Ⅲ～Ⅳ期组的凋亡阳性率和Fas阳性率均明显高于库肯勃氏瘤组(P＜0.01).Ⅰ～Ⅱ期组患者腹水T淋巴细胞凋亡与血清CA125水平呈正相关(r=0.77,P=0.009).血清CA125＞500 KU/L的卵巢癌患者腹水T淋巴细胞的凋亡高于CA125≤500 KU/L的患者(P=0.009).结论 (1)T淋巴细胞凋亡上调出现于较早期卵巢癌患者的腹腔局部和晚期患者的外周血,而且晚期卵巢癌腹水T淋巴细胞凋亡和Fas表达高于库肯勃氏瘤患者,反映了原发卵巢癌腹腔局部免疫缺陷的特殊性.(2)有效的治疗可以降低卵巢癌患者外周血T淋巴细胞的凋亡.(3)卵巢癌腹水T淋巴细胞的Fas表达显著上调,是卵巢肿瘤免疫治疗的重要靶点.(4)CA125能间接反映卵巢癌腹腔局部的免疫状况.
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