Impact of hydrogen sulfide, a novel gaseous signal molecule on nitric oxide/nitric oxide synthase pathway in left-to-right shunt: experiment with rats
- Author:
- No author available
- Journal Title:
- NATIONAL MEDICAL JOURNAL OF CHINA
- Issue:
- 19
- DOI:
- 10.3760/j:issn:0376-2491.2006.19.008
- Key Word:
- 高血压,肺性;硫化氢;心脏缺损,先天性;心血管畸形;一氧化氮合酶
Abstract： 目的探讨内源性硫化氢(H2S)对左向右分流大鼠一氧化氮(NO)/一氧化氮合酶(NOS)体系的影响.方法将雄性SD大鼠随机分为分流组,分流+炔丙基甘氨酸(PPG)组,假手术组和假手术组+PPG组,每组8只.分流组及分流+PPG组大鼠经腹主动脉-下腔静脉穿刺建立左向右分流动物模型.术后4周,测量大鼠平均肺动脉压(MPAP);测定血浆NO、肺组织H2S、NO含量及NOS活性;用Western印迹方法测定肺组织eNOS含量.结果分流术后4周,分流组与假手术组比较,大鼠MPAP无明显变化;分流组与假手术组比较血浆NO(23.2μmol/L±3.6 μmol/L vs 17.9μmol/L±3.4 μmol/L,P＜0.05)、肺组织H2S(37.6μmol/mg±2.1 μmol/mg vs 14.4μmol/mg±1.8μmol/mg,P＜0.05)、肺组织NO(38.5±6.5 μmol/μg vs 31.8±6.5μmol/μg,P＜0.05)、肺组织NOS活性(15.1 U/mg±2.4 U/mg vs 12.0 U/mg±1.4 U/mg,P＜0.05)及肺组织eNOS含量(0.3±0.1 vs0.2±0.1,P＜0.05)均明显升高.分流+PPG组大鼠MPAP较分流组及假手术组分别升高了15.82%和20.55%(19.5 mmHg±1.7 mm Hg vs 16.4 mmHg±1.7 mmHg和19.5 mmHg±1.7 mmHgvs 15.5 mmHg±1.3 mmHg,P＜0.05),肺组织H2S含量降低(28.8 μmol/mg±2.2 μmol/mg vs 37.6μmol/mg±2.1 μmol/mg,P＜0.05),而血浆NO(27.8 μmol/L±4.8 μmol/L vs 23.2μmol/L±3.6μmol/L,P＜0.05)、肺组织NO(46.0 μmol/μg±6.0 μmol/μg vs 38.5 μmol/μg±6.5μmol/μg,P＜0.05)、NOS活性(20.9 U/mg±3.9 U/mg vs 15.1 U/mg±2.4 U/mg,P＜0.05)及eNOS含量均明显升高(0.4±0.1 vs 0.3±0.1,P＜0.05).结论内源性H2S可能通过抑制NO/NOS体系调节左向右分流大鼠肺动脉压力.
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