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Effects of interleukin-18 on asthmatic airway inflammation: experimental study of guinea pig asthmatic model
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- Author:
- No author available
- Journal Title:
- NATIONAL MEDICAL JOURNAL OF CHINA
- Issue:
- 28
- DOI:
- 10.3760/j:issn:0376-2491.2005.28.013
- Key Word:
- 白细胞介素18;哮喘;炎症;趋化细胞因子类
Abstract： 目的观察白细胞介素18(IL-18)对豚鼠哮喘模型气道炎症的作用.方法采用卵蛋白(OVA)腹腔注射致敏加雾化吸入激发的方法复制豚鼠哮喘模型.30只豚鼠按随机数字法分成3组(每组10只)进行处理,分别为哮喘模型组(A组)、模型对照组(B组)和IL-18干预组(C组).光镜下检测各组豚鼠支气管肺泡灌洗液(BALF)中炎症细胞计数及分类,用酶联免疫吸附法测定BALF中Th1细胞因子IFN-γ、IL-2和Th2细胞因子IL-4、IL-5浓度,并进行3组之间的比较.结果豚鼠BALF中嗜酸粒细胞(EOS)个数A组、B组、C组分别为(98±58)×106/L、(12±10)×106/L、(29±10)×106/L,A组与B组、C组比较差异有统计学意义(P<0.01);中性粒细胞个数A组与B组、C组比较差异有统计学意义(P均<0.05).IFN-γ和IL-2浓度A组与B组比较差异有统计学意义(P<0.05),与C组比较差异亦有统计学意义(P均<0.01).IL-4浓度A组与B组、C组比较差异有统计学意义(P均<0.05);IL-5浓度A组与B组、C组比较差异有统计学意义(P<0.01).结论 IL-18可通过调节Th1/Th2细胞因子的平衡而达到控制哮喘气道炎症的作用.
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