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Role of connective tissue growth factor in angiotensin Ⅱ induced human proximal tubular cell hypertrophy
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- Author:
- No author available
- Journal Title:
- NATIONAL MEDICAL JOURNAL OF CHINA
- Issue:
- 17
- DOI:
- 10.3760/j:issn:0376-2491.2004.17.020
- Key Word:
- 肾小管;上皮细胞;血管紧张素Ⅱ;肥大;结缔组织生长因子
Abstract： 目的探讨结缔组织生长因子(CTGF)在介导血管紧张素Ⅱ( AngⅡ)诱导的人肾近端小管细胞肥大中的作用.方法用[3H]-亮氨酸掺入、考马斯亮蓝蛋白质定量技术分别观察抗CTGF抗体对AngⅡ诱导的人肾近端小管上皮细胞株(HK-2)细胞内蛋白质从头合成及细胞内蛋白质含量的影响;用流式细胞分析技术观察抗CTGF抗体对AngⅡ诱导的细胞周期分布改变的影响;用扫描电镜技术观察抗CTGF抗体对AngⅡ诱导细胞形态变化的影响.结果 AngⅡ可以显著诱导细胞内[3H]-亮氨酸掺入和细胞内总蛋白质含量增加的作用,呈浓度和时间依赖性;抗CTGF抗体对AngⅡ诱导的细胞内总蛋白质含量、[3H]-亮氨酸掺入量的增加有抑制作用,呈浓度和时间依赖性.AngⅡ作用于细胞48 h后,细胞平均直径、G0-G1期细胞百分比显著增加(均P<0.01),抗CTGF抗体可显著抑制AngⅡ诱导的细胞直径增加和细胞周期阻滞 (均P<0.05或P<0.01).结论 AngⅡ可以诱导肾小管上皮细胞发生肥大, CTGF可能介导了AngⅡ诱导的肾小管上皮细胞肥大效应.
- [1]刘必成,罗冬冬,孙子林,等.结缔组织生长因子在糖尿病大鼠肾脏中的表达及其意义[J].2002,(1).
- [2]赵青,陈楠,王伟铭,等.结缔组织生长因子在肾间质纤维化中的表达及其意义[J].2002,(1).
- [3]R.A. Risdon,J.C. Sloper,H.E. De Wardener.Relationship Between Renal Function and Histological Changes Found in Renal-Biopsy Specimens from Patients with Persistent Glomerular Nephritis[J].1968,292(7564).
- [4]Zeisberg M,Muller GA,Strutz F.Renal fibrosis: an update.[J].2001,10(3).
- [5]刘必成,陈琪,罗冬冬,等.伊贝沙坦预防链脲佐菌素诱导糖尿病大鼠肾损伤的作用机制[J].2003,(1).
- [6]Aten J,Oemar BS,Ito Y,etc.Expression of connective tissue growth factor in human renal fibrosis.[J].1998,53(4).
- [7]Wahab NA,Weston BS,Mason RM,etc.Connective tissue growth factor and regulation of the mesangial cell cycle: role in cellular hypertrophy.[J].2002,13(10).
- [8]Wolf G,Ziyadeh FN.Molecular mechanisms of diabetic renal hypertrophy.[J].1999,56(2).
- [9]Chand M,Kleij L,Bende R,etc.Kinetics of connective tissue growth factor expression during experimental proliferative glomerulonephritis.[J].2001,12(3).
- [10]Wolf G,Neilson EG.Morphogenic cues that modulate podocyte growth (editorial; comment)[J].1998,53(4).
- [11]T M, Kennefick,S, Anderson.Role of angiotensin II in diabetic nephropathy.[J].1997,17.