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Nanoparticle as a new gene transferring vector in specific expression gene
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- Author:
- No author available
- Journal Title:
- NATIONAL MEDICAL JOURNAL OF CHINA
- Issue:
- 5
- DOI:
- 10.3760/j:issn:0376-2491.2002.05.016
- Key Word:
- 基因治疗;载体;纳米粒子;反义单核细胞趋化因子;血管再狭窄
Abstract： 目的观察纳米粒子携带特异性基因转染的可行性及其效率.方法应用聚乳酸聚乙醇酸共聚物和聚乙烯醇包载特异性反义单核细胞趋化蛋白-1基因,制备纳米级粒子混合物.检测其包埋率、体外释放情况及粒度.以阳离子脂质体为对照,利用培养的平滑肌细胞,应用聚合酶链反应(PCR)观察其为真核细胞传递基因的可能性;采用移植静脉内膜增生动物模型,应用RNA斑点杂交和病理形态学分析,观察其在体内的基因转染、表达及生物学效应.结果制备的纳米粒子-DNA粒度为150～300 nm,包埋率为:0.9%,体外释放时间为2周左右.PCR结果提示:纳米粒子可将目的基因转移至平滑肌细胞中.体内实验显示:纳米粒子可实现体内局部基因转染、表达,发挥相应的效应.结论纳米粒子可用于特异性基因的转染.
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