The electrophysiological properties of voltage-gated potassium channels on cultivated porcine retinal ganglion cells irradiated with continuous near-infrared laser
- Author:
- No author available
- Journal Title:
- Chinese Journal of Ophthalmology
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.0412-4081.2012.02.012
- Key Word:
- 视网膜神经节细胞;细胞,培养的;膜片钳术;钾通道;谱学,近红外线;Retinal ganglion cells;Cells,cultured;Patch-clamp techniques;Potassium channels;Spectroscopy,near-infrared
Abstract： Objective To investigate the influence of continuous near-infrared (CNI) laser to potassium ion channels on retinal ganglion cell ( RGC).Methods Experiment study.Porcine RGC was cultured with enzymatic digestion method in vitro by taking off the retina from the piglets.Whole-cell patch clamp mode recordings were obtained from primary cultured porcine RGC.Whole-cell currents of porcine RGC irradiated with a single-mode CNI laser of 845 nm wavelength and 30 mW power were also recorded.The primary cultured porcine RGC were divided into laser irradiation group and control group according to whether they accepted CNI laser of 845 nm wavelength.t test was used to analyze the average peak amplitude between the two groups.Results The cells had the morphological characteristics of typical neurons after one week cultured observed by inverted phase contrast microscope.The body and bumps with yellow-green fluorescence was positive cell which observed by immune cell fluorescence chemical testing.CNI laser had a regulation on outward potassium current of porcine RGC in a voltage-dependent manner.There was significant difference on the average peak amplitude of potassium current between the laser irradiation group [ (634.4 ± 86.8 ) Pa]and the control group [ ( 580.5 ± 116.4 ) Pa ],respectively ( n =30,t =7.923,P =0.000).Conclusions CNI laser can change the properties of outward K + channel.Therefore,formation and releasing of action potential is affected.Further,physiological functions of RGC are regulated,which might contribute to the protection and restoration of injured RGC.It can be provided a new scholar direction for the protection of the RGC,which are injured by glaucoma.
- [1]宓现强,岑剡,周正谊,等.低强度激光照射对离体动物红细胞流变学性质的影响[J].2004,(7).
- [2]白海青,王竫华,王大博,等.新生大鼠视网膜神经元及节细胞体外短期培养方法[J].2004,(2).
- [3]刘文文,徐萍,黄倩.胚胎与成人视网膜神经细胞培养[J].2002,(4).
- [4]骆晓森,倪晓武,陆建,等.不同波长光照射血液诱发的荧光光谱研究[J].2003,(1).
- [5]曲超,乔利峰,曹伟,等.670 nm近红外光对大鼠视网膜光损伤的保护作用[J].2009,(2).
- [6]Margolis D,Whelan HT,Hodgson BD,etc.Clinical and experimental applications of NIR-LED photobiomodulation.[J].2006,24(2).
- [7]Lim J,Whelan HT,Millsap DS,etc.Effects of 670-nm phototherapy on development.[J].2005,23(3).
- [8]Niven JE,Juusola M,Vahasoyrinki M,etc.Robustness of neural coding in Drosophila photoreceptors in the absence of slow delayed rectifier K+ channels.[J].2006,26(10).
- [9]Whelan HT,Watkins-JB 3rd,Sanders RA,etc.670 Nanometer Light Treatment Attenuates Dioxin Toxicity in the Developing Chick Embryo.[J].2006,20(6).
- [10]Xu ZC,Chi XX.Differential changes of potassium currents in CA1 pyramidal neurons after transient forebrain ischemia.[J].2000,84(6).
- [11]De Castro Rodrigues A,Gigo Benato D,Tos P,etc.Low-power laser biostimulation enhances nerve repair after end-to-side neurorrhaphy: a double-blind randomized study in the rat median nerve model.[J].2004,19(1).
- [12]F J, Seil.The extracellular matrix molecule, laminin, induces purkinje cell dendritic spine proliferation in granule cell depleted cerebellar cultures.[J].1998,795(1-2).
- [13]Tiina Karu.Primary and secondary mechanisms of action of visible to near-IR radiation on cells[J].1999,49(1).
- [14]Pretorius AM,Whelan HT,Ciftcioglu N,etc.A preliminary investigation into light-modulated replication of nanobacteria and heart disease.[J].2003,21(4).
- [15]Whelan HT,Dhokalia A,Das R,etc.Effect of NASA light-emitting diode irradiation on molecular changes for wound healing in diabetic mice.[J].2003,21(2).
- [16]Anders JJ,Rochkind S,Geuna S.Phototherapy promotes regeneration and functional recovery of injured peripheral nerve.[J].2004,26(2).
- [17]T. I. Karu,T. P. Ryabykh,G. E. Fedoseyeva,etc.Helium-neon laser-induced respiratory burst of phagocytic cells[J].1989,9(6).
- [18]Whelan NT,Whelan HT,Kane M,etc.Therapeutic photobiomodulation for methanol-induced retinal toxicity.[J].2003,100(6).
- [19]E, Neher,B, Sakmann.Single-channel currents recorded from membrane of denervated frog muscle fibres.[J].1976,260(5554).
- [20]Janis T. Eells,Ellen V. Buchman,Lisa J. Gould,etc.Mitochondrial signal transduction in accelerated wound and retinal healing by near-infrared light therapy[J].2004,4(5).
- [21]Basford JR.Low intensity laser therapy: still not an established clinical tool.[J].1995,16(4).
- [22]Luo X,Lambrou GN,Sahel JA,etc.Hypoglycemia induces general neuronal death, whereas hypoxia and glutamate transport blockade lead to selective retinal ganglion cell death in vitro.[J].2001,42(11).
- [23]Robbins J,Lilley S.The rat retinal ganglion cell in culture: an accessible CNS neurone.[J].2005,51(3).
- [24]Whelan HT,Barbeau L,Post AC,etc.NASA light-emitting diodes for the prevention of oral mucositis in pediatric bone marrow transplant patients.[J].2002,20(6).
- [25]Endre Mester,Andrew F. Mester,Adam Mester.The biomedical effects of laser application[J].1985,5(1).
- [26]Key B,Tisay KT.The extracellular matrix modulates olfactory neurite outgrowth on ensheathing cells.[J].1999,19(22).
- [27]Cwiklinski J,Philippi AF,Cevenini V,etc.Effect of NASA light-emitting diode irradiation on wound healing.[J].2001,19(6).
- [28]S.A. Jo,E. Wang,L.I. Benowitz.Ciliary neurotrophic factor is an axogenesis factor for retinal ganglion cells[J].1999,89(2).