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Effect of genistein on proliferation of rabbit lens epithelial cell
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OPHTHALMOLOGY
- Issue:
- 7
- DOI:
- 10.3760/j:issn:0412-4081.2002.07.013
- Key Word:
- 晶体;上皮细胞;受体蛋白质酪氨酸激酶;染料木黄酮
Abstract： 目的研究受体蛋白质酪氨酸激酶(receptor protein-tyrosine kinase, RPTK)抑制剂染料木黄酮对兔晶状体上皮细胞增生和胞膜RTPK活性的影响,探讨使用染料木黄酮防治后发性白内障发生的机制.方法培养兔晶状体上皮细胞;测定5、10和15 mg/L 染料木黄酮作用晶状体上皮细胞3 d的氚标记胸腺嘧啶脱氧核苷(tritiatde thymidine, 3H-TdR)掺入率,以无染料木黄酮作用的晶状体上皮细胞作为对照组;应用Casnetllie改良法检测不同浓度染料木黄酮作用24 h后,兔晶状体上皮细胞膜RTPK活性的变化.结果 5、10和15 mg/L 染料木黄酮作用晶状体上皮细胞3 d的3H-TdR掺入率分别为630±137、489±166和314±98,与对照组比较差异均有显著意义(P<0.05),且细胞增生抑制率随浓度增加而升高;不同浓度染料木黄酮作用24 h后兔晶状体上皮细胞中RTPK的活性均明显下降,其中10和15 mg/L 染料木黄酮作用的晶状体上皮细胞RTPK单位活性与无染料木黄酮作用者比较,差异均有显著意义(P<0.05).结论染料木黄酮在体外可通过抑制细胞膜RTPK的活性而抑制兔晶状体上皮细胞增生.
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