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Expression of mRNA for proteins involved in retinol metabolism in cultured bovine iris pigment epithelial cells vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OPHTHALMOLOGY
- Issue:
- 6
- DOI:
- 10.3760/j:issn:0412-4081.2002.06.013
- Key Word:
- 虹膜;色素上皮,眼;细胞培养;维生素A;逆转录聚合酶链反应
Abstract： 目的评价体外培养牛眼虹膜色素上皮(iris pigment epithelial IPE)细胞的维生素A代谢功能.方法采用酶辅助的显微分离法和培养新生牛眼IPE细胞,Trizol 一步法提取总RNA。根据Genbank中维生素A结合蛋白(retinol-binding prtein ,RBP)和细胞内视醛结合蛋白(cellular retinldehydebinding protein,CRALBKP)的相关系列设计特异引物,对其mRNA的表达进行逆录聚合酶链反应(reverse transcription polymerase-chain reaction reaction ,RT-PCR)检测.结果传2代牛眼IPE细胞中提取的总RNA完整、无降解.RT-PCR检测结果:RBP及CRALBP的mRNA在培养的牛眼IPE细胞中均有表达.结论牛眼IPE细胞具有与视网膜色素上皮细胞相似的维生素A转运和代谢功能.
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