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Urea soluble fraction of bovine melanin associated antigen and experimental study on its uveitogenic activity
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OPHTHALMOLOGY
- Issue:
- 3
- DOI:
- 10.3760/j:issn:0412-4081.2002.03.012
- Key Word:
- 脲溶性牛黑素相关抗原;葡萄膜炎;变态反应和免疫学;疾病模型,动物
Abstract： 目的探讨眼葡萄膜组织中致葡萄膜炎活性的自身抗原.方法采用生化方法提取牛虹膜睫状体中脲溶性牛黑素相关抗原,对其进行凝胶电泳分析、检测氨基酸组成成分及含量;用脲溶性牛黑素相关抗原加佐剂对F344大鼠和Lewis大鼠进行免疫,诱发实验性脲溶性牛黑素相关抗原诱导的自身免疫性葡萄膜炎(experimental urea soluble farction of the bovine melanin-associated-antigen-induced uveitis, EMIU),并观察脲溶性牛黑素相关抗原的生物活性.结果脲溶性牛黑素相关抗原相对分子质量约为64 000,含有17种氨基酸,可诱发F344大鼠和Lewis大鼠的EMIU, 炎性反应主要发生在前葡萄膜,严重者可伴发局灶性脉络膜炎,不伴有视网膜炎和松果体炎.结论脲溶性牛黑素相关抗原可能是葡萄膜组织中致葡萄膜炎活性的主要自身抗原,其诱发的EMIU有别于视网膜可溶性抗原所诱导的实验性自身免疫性葡萄膜视网膜炎,不伴视网膜炎和松果体炎.
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