Abstract： Objective To analyze epidemiological characteristics of acute hepatitis B (aHPB) in Beijing and to recheck epidemiology and laboratory results of aHPB cases in the sampling area.Methods We analyzed epidemiological characteristics of aHPB cases reported in National Notifiable Disease Reporting System (NNDRS) from 2005 to 2014 in Beijing,using a stratified cluster sampling method and based on the average reported incidence of hepatitis B from 2005 to 2011 in NNDRS,we projected 430 aHPB cases in the sampling area from 2012 to 2014,for which 307 received blood sample retesting.Inclusion criteria of aHPB cases were those living in Beijing more than 1 year,using name,sex,age,or identity card number as key fields to verify that they had not been previously reported in the database.Accuracy and adjusted aHPB incidence rate for 2005-2014 in Beijing was analyzed by rechecking aHPB cases.Results Epidemiological survey showed that aHPB coincidence rate was 89.5％ (385/430) in NNDRS from 2012 to 2014 in Beijing.Laboratory tests showed that the aHPB coincidence rate was 92.2％ (283/307).The total coincidence rate was 82.54％.The aHPB adjustment incidence rate dropped from 5.05/100 thousand in 2005 to 0.98/100 thousand in 2014 in Beijing.The aHPB adjustment incidence rate among residents younger than 15 years dropped to 0/100 thousand.Conclusion The incidence of aHPB in Beijing dropped to a low level from 2005 to 2014.There were no aHPB cases in residents younger than 15 years.