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Studies on DNA damage and apoptosis in rat brain induced by fluoride
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PREVENTIVE MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/j:issn:0253-9624.2002.04.002
- Key Word:
- 氟化钠;脑;DNA损伤;脱噬作用;流式细胞术
Abstract： 目的探讨氟对大鼠脑细胞DNA的损伤作用及诱导凋亡的作用.方法体内试验中,对照组大鼠腹腔注射蒸馏水,氟组大鼠注射氟化钠,染毒剂量为20 mg*kg-1*d-1;体外试验中,采用5 mmol/L氟化钠对急性分离的大脑神经细胞染毒2 h后用单细胞凝胶电泳技术(SCGE)研究氟对细胞DNA的损伤(单链断裂).采用TUNEL法和流式细胞术(FCM)检测细胞凋亡.结果体内试验氟组大鼠大脑皮层神经细胞DNA损伤情况明显比对照组严重,Ridit值分别为0.351和0.639,体外试验对照组与氟组Ridit值分别为0.650 1和0.384 4.TUNEL阳性细胞在氟组大鼠大脑皮层、海马及小脑颗粒细胞层均可见到,在对照组大鼠则很罕见.流式细胞术检测结果表明大脑皮层及海马神经细胞凋亡百分率氟组(27.12±3.08,34.97±5.46)均高于对照组(4.63±0.98,5.35±0.79),差异有极显著性意义.结论氟化钠可引起大鼠脑细胞DNA损伤和细胞凋亡发生.
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