Paper - WANFANG MED ONLINE

You Position: Home > Paper

Quantitative proteomic analysis of the retina in the rat model of non-arteritic anterior ischemic optic neuropathy

( views:184, downloads:93 )

Author:: No author available

Journal Title:: Chinese Journal of Ocular Fundus Diseases

Issue:: 3

DOI:: 10.3760/cma.j.cn511434-20201124-00522

Key Word:: 视神经病变，缺血性;视神经;视网膜;蛋白质组学;Optic neuropathy, ischemic;Optic nerve;Retina;Proteomics

Abstract： Objective:To analyze the protein expression changes in the retina of non-arteritic anterior ischemic optic neuropathy (NAION) in rats.Methods:The rat NAION (rNAION) model was established by Rose Bengal and laser. Twenty Sprague-Dawley rats were randomly divided into 4 groups, the normal control group, the laser control group, the RB injection control group, and the rNAION model group, with 5 rats in each group. The right eye was used as the experimental eye. The retina was dissected at the third day after modeling. Enzyme digestion method was used for sample preparation and data collection was performed in a non-dependent collection mode. The data were quantitatively analyzed by SWATH quantitative mass spectrometry, searching for differential proteins and performing function and pathway analysis.Results:Compared with the other three control groups, a total of 184 differential proteins were detected in the rNAION group (expression fold greater than 1.5 times and P<0.05), including 99 up-regulated proteins and 85 down-regulated proteins. The expressions of glial fibrillary acidic protein, guanine nucleotide binding protein 4, laminin 1, 14-3-3γ protein YWHAG were increased. Whereas the expressions of Leucine-rich glioma-inactivated protein 1, secretory carrier-associated membrane protein 5, and Clathrin coat assembly protein AP180 were decreased. The differential proteins are mainly involved in biological processes such as nerve growth, energy metabolism, vesicle-mediated transport, the regulation of synaptic plasticity, apoptosis and inflammation. Pathway enrichment analysis showed that PI3K-Akt signaling pathway and complement and thrombin reaction pathway was related to the disease. Conclusion:The protein expressions of energy metabolism, nerve growth, synaptic vesicle transport and PI3K-Akt signaling pathway can regulate the neuronal regeneration and apoptosis in NAION.

Reference

[1]肖静,张晓敏,李筱荣.糖尿病视网膜病变的蛋白质组学研究进展[J].2018,(4).
[2]王一玮,陈婷,马瑾,等.大鼠非动脉炎性前部缺血性视神经病变模型RGC及视神经损伤的特点[J].2016,(12).
[3]Linde, Kegel,Eerik, Aunin,Dies, Meijer,etc.LGI proteins in the nervous system.[J].2013,5(3).
[4]Masoud Aghsaei Fard,Sara Fakhree,Ahmad Ameri.Posterior Pole Retinal Thickness for Detection of Structural Damage in Anterior Ischaemic Optic Neuropathy[J].2013,37(5).
[5]GuanXiong,Ye,Yong,Qin,Shi,Wang,etc.Lamc1 promotes the Warburg effect in hepatocellular carcinoma cells by regulating PKM2 expression through AKT pathway.[J].2019,20(5).
[6]Hubert, Laurence,Serio, Magda Cannata,Villoing-Gaude, Laure,etc.De novo SCAMP5 mutation causes a neurodevelopmental disorder with autistic features and seizures[J].2020,57(2).
[7]Jagriti, Pal,Vikas, Patil,Baisakhi, Mondal,etc.Epigenetically silenced GNG4 inhibits SDF1α/CXCR4 signaling in mesenchymal glioblastoma.[J].2016.
[8]Y-W, Chu,C-R, Wang,F-B, Weng,etc.MicroRNA-222 contributed to cell proliferation, invasion and migration via regulating YWHAG in osteosarcoma.[J].2018,22(9).
[9]Whitmore, S. Scott,Tatro, Nicole,Novbatova, Gulnara,etc.Analysis of 14-3-3 isoforms expressed in photoreceptors[J].2018,170.
[10]Tanigami A,Suzuki M,Takahashi E,etc.Cloning, expression, and chromosomal mapping of the human 14-3-3gamma gene (YWHAG) to 7q11.23.[J].1999,60(2).
[11]Guo Y,Miller NR,Zhang C.Optic nerve infarction and post-ischemic inflammation in the rodent model of anterior ischemic optic neuropathy (rAION).[J].2009,1264.
[12]Huang, Qiang,Yang, Lu,Luo, Ji,etc.SWATH enables precise label-free quantification on proteome scale[J].2015,15(7).
[13]Abu Amero KK,Bosley TM.Increased relative mitochondrial DNA content in leucocytes of patients with NAION.[J].2006,90(7).
[14]Yuh-Man, Sun,Megan, Cooper,Sophie, Finch,etc.Rest-mediated regulation of extracellular matrix is crucial for neural development.[J].2008,3.
[15]Huang Yao,Li Zhiwei,Wang Ningli,etc.Roles of PI3K and JAK pathways in viability of retinal ganglion cells after acute elevation of intraocular pressure in rats with different autoimmune backgrounds[J].2008,9(1).
[16]Roth L,Roussel G,Bagnard D,etc.Functional interaction between matrix metalloproteinase-3 and semaphorin-3C during cortical axonal growth and guidance.[J].2007,17(7).
[17]L A, Levin,A, Louhab.Apoptosis of retinal ganglion cells in anterior ischemic optic neuropathy.[J].1996,114(4).
[18]Flower RW,Guo Y,Kelman SE,etc.Functional and cellular responses in a novel rodent model of anterior ischemic optic neuropathy.[J].2003,44(10).
[19]Gajanan,Sathe,Chan Hyun,Na,Santosh,Renuse,etc.Quantitative proteomic profiling of cerebrospinal fluid to identify candidate biomarkers for Alzheimer's disease.[J].2018.
[20]Zhang C,Guo Y,Slater BJ,etc.Axonal degeneration, regeneration and ganglion cell death in a rodent model of anterior ischemic optic neuropathy (rAION).[J].2010,91(2).
[21]Nehal M,Elsherbiny,Yousra,Abdel-Mottaleb,Amany Y,Elkazaz,etc.Carbamazepine Alleviates Retinal and Optic Nerve Neural Degeneration in Diabetic Mice via Nerve Growth Factor-Induced PI3K/Akt/mTOR Activation.[J].2019,13.
[22]Chen, Xueli,Rao, Jun,Zheng, Zhi,etc.Integrated Tear Proteome and Metabolome Reveal Panels of Inflammatory-Related Molecules via Key Regulatory Pathways in Dry Eye Syndrome[J].2019,18(5).
[23]Leguern E,Wang X,Zhou YD,etc.Epilepsy gene LGI1 regulates postnatal developmental remodeling of retinogeniculate synapses.[J].2012,32(3).
[24]Ludovic C. Gillet,Pedro Navarro,Stephen Tate,etc.Targeted Data Extraction of the MS/MS Spectra Generated by Data-independent Acquisition: A New Concept for Consistent and Accurate Proteome Analysis[J].2012,11(6).
[25]Mehrabian Z,Guo Y,Slater BJ,etc.Rodent anterior ischemic optic neuropathy (rAION) induces regional retinal ganglion cell apoptosis with a unique temporal pattern.[J].2008,49(8).
[26]Downes GB,Gautam N.The G protein subunit gene families.[J].1999,62(3).
[27]Selevsek, Nathalie,Chang, Ching-Yun,Gillet, Ludovic C.,etc.Reproducible and Consistent Quantification of the Saccharomyces cerevisiae Proteome by SWATH-mass spectrometry[J].2015,14(3).
[28]Zhao,H.,Kim,Y.,Park,J.,etc.SCAMP5 Plays a critical role in synaptic vesicle endocytosis during high neuronal activity[J].2014,34(30).
[29].売上が伸びなくても利益確保--12年度海外売上700億円、2千人を減員[J].2008,(1919).
[30]Kyong Jin Cho,Hae Young Lopilly Park,Chan Kee Park,etc.Glial cell response and iNOS expression in the optic nerve head and retina of the rat following acute high IOP ischemia-reperfusion[J].2011,1403.
[31]Tang, Fen,Xu, Fan,Cui, Ling,etc.The expression and role of PIDD in retina after optic nerve crush[J].2020,51(1).
[32]Sebastian Funke,Natarajan Perumal,Katharina Bell,etc.The potential impact of recent insights into proteomic changes associated with glaucoma[J].2017,14(4).
[33]Yvonne, Bouter,Tim, Kacprowski,Robert, Weissmann,etc.Deciphering the molecular profile of plaques, memory decline and neuron loss in two mouse models for Alzheimer's disease by deep sequencing.[J].2014,6.
[34]Cristian, Salgado,Fernandino, Vilson,Neil R, Miller,etc.Cellular inflammation in nonarteritic anterior ischemic optic neuropathy and its primate model.[J].2011,129(12).
[35]Fusco,C.,Micale,L.,Augello,B.,etc.Smaller and larger deletions of the Williams Beuren syndrome region implicate genes involved in mild facial phenotype, epilepsy and autistic traits[J].2014,22(1).
[36]D, Morrison.14-3-3: Modulators of Signaling Proteins?[J].1994,266(5182).
[37]María, Nicolás-Pérez,Franz, Kuchling,Joaquín, Letelier,etc.Analysis of cellular behavior and cytoskeletal dynamics reveal a constriction mechanism driving optic cup morphogenesis.[J].2016,(Spec ).
[38]Fan YM,Luo JM,Chiang SW,etc.PI3K/akt, JAK/STAT and MEK/ERK pathway inhibition protects retinal ganglion cells via different mechanisms after optic nerve injury.[J].2007,26(4).
[39]Feilchenfeld Z,Yucel YH,Gupta N.Oxidative injury to blood vessels and glia of the pre-laminar optic nerve head in human glaucoma.[J].2008,87(5).
[40]Cornell, Brett,Wachi, Tomoka,Zhukarev, Vladimir,etc.Overexpression of the 14-3-3gamma protein in embryonic mice results in neuronal migration delay in the developing cerebral cortex[J].2016,628.
[41]Bonham, Luke W.,Evans, Daniel S.,Liu, Yongmei,etc.Neurotransmitter Pathway Genes in Cognitive Decline During Aging: Evidence for GNG4 and KCNQ2 Genes[J].2018,33(3).
[42]McKee,K.K.,Yang,D.-H.,Patel,R.,etc.Schwann cell myelination requires integration of laminin activities[J].2012,125(19).
[43]Ramocki MB,Bartnik M,Szafranski P,etc.Recurrent distal 7q11.23 deletion including HIP1 and YWHAG identified in patients with intellectual disabilities, epilepsy, and neurobehavioral problems.[J].2010,87(6).