Abstract： Objective To investigate the difference in microperimetry between acute and chronic central serous chorioretinopathy (CSC).Methyds Cross-sectional cases study.A consecutive series of 208 patients (221 eyes) with CSC diagnosed by fundus fluorescein angiography (FFA) and optical coherence tomography (OCT) were enrolled in the study.The patients were divided into acute group (136 patients,143 eyes) and chronic group (72 patients,78 eyes) according to the duration and FFA.There were no statistical difference in sex (x2 =0.012,P=0.912) and mean age (t=-1.492,P=0.137) between two groups.All eyes received the examination of microperimetry and minimum resolution angle in logarithmic (logMAR) best corrected visual acuity (BCVA).The mean retinal sensitivities (MS) and fixation rate in the central 2°(P1)and 4° (P2) were determined.Results The mean value of logMAR BCVA in acute group and chronic group were 0.32 ± 0.23 and 0.48 ± 0.33,there was significant difference (Z=-3.353,P =0.001).In acute group and chronic group,the MS were (21.25±5.06) and (15.82±7.23) dB,P1 were (76.36±25.78)％ and (55.01±32.34)％,P2 were (92.21±13.06)％ and (79.83±23.11)％.There were statistical differences in MS (Z=-5.456,P＜0.001),P1 (Z=-4.629,P＜0.001) and P2 (Z=-4.265,P＜0.001) between two groups.In acute group,fixation was stable in 98 eyes (68.5％),relative unstable in 30 eyes (21.0 ％),unstable in 15 eyes (10.5 ％).In chronic group,fixation was stable in 30 eyes (38.5％),relative unstable in 22 eyes (28.2％),unstable in 26 eyes (33.3％).The difference of fixation between two groups was statistically significant (x2 =23.196,P＜0.001).Conclusion MS,fixation rate and fixation stability in chronic CSC eyes were all decreased compared with acute CSC eyes.