Abstract： Objective To observe the effects of structure and function of cornea,chamber angle and retina of varying doses of Bevacizumab which was injected intravitreally in rabbits. Methods Twenty-four New Zealand albino rabbits were divided into three groups randomly,the right eyes in three groups received intravitreal injection Avastin at dose 1.25 mg,2.5 mg and 5 mg respectively as experimental eye.the left eyes accepted intravitreal injection 0.9%normal saline at the same volume as a control eye.The anterior segment of eye and ocular fundus were examined and intraocular pressure was measured by slit-lamp microscope and direct ophthalmoscope before and after injection.It was tested by Electroretinogram(ERG)before and after injection 1,4,8 weeks.At the 8th week,it carried out corneal endothelium counting;then enucleated eyes tO observe by the light microscope and transmission electron microscope. Results No statistically significant difference regard to IOP,corneal endothelium counting,a-and b-waves of ERG at any stage of study in every group(P＞0.05).No obvious change at cornea,chamber angle.retinal structure and retinal uhrastructure in every group under light microscope.Conclusion This study indicated that there is no obvious toxicity of intravitreal injection with Avastin 1.25～5.0 mg in normal rabbit eyes.