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Pathological mechanism of experimental retinal detachment induced by hepatocyte growth factor
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 3
- DOI:
- 10.3760/j.issn:1005-1015.2007.03.011
- Key Word:
- 视网膜脱离/病因学;肝细胞生长因子;疾病模型,动物
Abstract： 目的 观察肝细胞生长因子(HGF)对视网膜色素上皮(RPE)细胞屏障功能的影响以及RPE内过度表达HGF导致视网膜脱离(RD)的病理机制.方法 编码HGF(AdCMV.HGF)、绿色荧光蛋白(Ad CMV.GFP)的E1/E3缺失的腺病毒载体,以5×104噬斑形成单位(pfu)/眼注射到成年有色兔的视网膜下.检查注射后3、7、14、28 d时的眼底及组织病理变化,利用免疫组织化学和酶联免疫吸附试验(ELISA)方法检测HGF在视网膜和玻璃体的表达水平.结果 对照组注射Ad CMV.GFP眼显示GFP几乎仅表达于PRE单核细胞层,AdCMV.HGF注射眼在注射点处的PRE细胞出现强的HGF免疫阳性反应.玻璃体内HGF的表达水平在注射7 d后达到最高峰、28 d后降低到基础水平.在HGF的表达期内AdCMV.HGF注射眼出现慢性RD和脉络膜慢性炎症.在RD区域,视网膜下的空间内可见增生性的RPE细胞,部分实验兔眼还产生多层的细胞膜结构.结论 RPE内过度表达的HGF能引发慢性浆液性RD,同时伴有视网膜下RPE增生.提示HGF可能作为治疗RD的作用靶点.
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