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Interference effect of nerve growth factor on apoptosis of retinal cells in experimental retinal detachment
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1005-1015.2006.05.013
- Key Word:
- 视网膜脱离;神经生长因子;细胞死亡;疾病模型,动物
Abstract： 目的 研究神经生长因子(NGF)在实验性视网膜脱离(RD)时对视网膜细胞凋亡的干预作用.方法 27只大鼠左、右眼分别被分为实验对照组和NGF组.视网膜下注射透明质酸钠建立RD动物模型后,取右眼在玻璃体腔内注射1 μg/μl NGF,共5μl,作为NGF组;左眼注射磷酸盐缓冲液(PBS)5μl,作为实验对照组.注射方法为每4天注射1次,直至观察期结束.两组在建立模型后1.5、3、6、12 h、1、2、4、8、16、32 d分别取眼球.另设立正常对照组2只大鼠,不作任何处理,在实验观察结束时取眼球.采用原位末端标记法(TUNEL)和透射电子显微镜观察视网膜细胞凋亡情况,并进行细胞计数和统计学检验.结果 RD早期就有细胞凋亡的发生.视网膜各层细胞均可见凋亡细胞,内、外核层分布最多.凋亡细胞数随脱离时间延长而增加(P＜0.01).NGF组的凋亡细胞数比实验对照组少(P＜0.01).结论 实验性RD中,玻璃体腔内给予外源性NGF能抑制视网膜细胞凋亡的发生.
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