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Effects of angiostatin on the activities of extra-cellular signal-regulated protein kinase in microvascular endothelial cells of rat's retina
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
- Issue:
- 3
- DOI:
- 10.3760/j.issn:1005-1015.2005.03.012
- Key Word:
- 视网膜新生血管化/病理生理学;血管生成抑制剂/药理学;血管抑素;有丝分裂激活蛋白激酶类
Abstract： 目的观察血管抑素对体外培养的鼠视网膜微血管内皮细胞的细胞外信号调节激酶(ERK-1)活性的影响.方法利用l-lysine耦联的sepharose 4B亲和层析柱从血浆中分离和纯化血管抑素.原代培养鼠视网膜微血管内皮细胞分为4组:对照组,血管内皮因子(VEGF)10 ng/ml组,血管抑素130 μg/ml组,VEGF(10 ng/ml)+血管抑素(130 μg/ml)组,Western-blotting检测血管抑素对血管内皮因子刺激后1、2、5、10、15、30 min的视网膜微血管内皮细胞ERK-1水平的影响. 结果与对照组相比,经血管抑素处理后1 min,ERK-1水平开始下降,10 min时下降最为显著,30 min后对照组与血管抑素组ERK水平没有显著区别.VEGF刺激后,鼠视网膜微血管内皮细胞中ERK-1被迅速激活,5 min后达到高峰,VEGF组ERK水平较对照组提高了210%(P＜0.05);30 min后,VEGF组ERK水平与对照组比较,差异无统计学意义(P＞0.05).而血管抑素能使ERK-1的表达量降低11.9%(1 min)、17.9%(2 min)、38.7%(5 min)、49.3%(10 min)(P＜0.05)、27.9%(15 min)、1.12%(30 min).结论血管抑素在体外能有效的阻断VEGF由细胞外向细胞核传导的信号通路.
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