You Position: Home > Paper
Influence of cold ischemia on liver regeneration following partial liver transplantation in rats
( views:145, downloads:12 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.0254-1785.2004.04.010
- Key Word:
- 肝移植;肝再生;肿瘤坏死因子-α;白细胞介素10
Abstract： 目的研究冷缺血对移植肝再生及肿瘤坏死因子(TNF-α)和白细胞介素10(IL-10)表达的影响.方法建立稳定的大鼠部分肝移植模型.实验分为:50%肝切除对照组、冷缺血30 min后部分肝移植组(实验1组)和冷缺血10 h后部分肝移植组(实验2组).观察各组生存率,并分别于术后1 d、2 d、4 d取肝组织,免疫组织化学检测各组增殖细胞核抗原(PCNA)、TNF-α、IL-10的表达;对各组肝再生增殖及TNF-α和IL-10的表达进行相关性分析;探讨TNF-α和IL-10的变化对大鼠部分肝移植术后肝再生的影响.结果50%肝切除组、冷缺血30 min后部分肝移植组和冷缺血10 h部分肝移植组存活率分别为100%,79%、29%;冷缺血10 h部分肝移植组与冷缺血30 min部分肝移植组相比,PCNA、TNF-α表达降低(P＜0.05),而IL-10表达增高(P＜0.05).结论随着冷缺血时间的延长,降低了部分肝移植术后的肝再生能力和生存率.TNF-α和IL-10在移植肝肝再生过程中起重要的调控作用.
- [1]李相成,王学浩,俞悦,等.大鼠部分肝移植的实验研究[J].2002,(3).
- [2]李相成,王学浩,俞悦,等.大鼠肝移植后肝再生的实验研究[J].2002,(4).
- [3]Ascher NL,Emond JC,Omura T.Fifty-percent partial liver transplantation in the rat.[J].1996,62(2).
- [4]Meijer S,Dijkstra CD,Schouten HJ,etc.Kupffer cell depletion by CI2MDP-liposomes alters hepatic cytokine expression and delays liver regeneration after partial hepatectomy.[J].2000,20(1).
- [5]Kasahara M,Uemoto S,Hayashi M,etc.Impact of graft size mismatching on graft prognosis in liver transplantation from living donors.[J].1999,67(2).
- [6]Rikiyama T,Oikawa M,Unno M,etc.Interleukin-10 induction after combined resection of liver and pancreas.[J].2001,48(42).
- [7]D F, Fiorentino,M W, Bond,T R, Mosmann.Two types of mouse T helper cell. IV. Th2 clones secrete a factor that inhibits cytokine production by Th1 clones.[J].1989,170.
- [8]Delgado-Rizo, V.,Salazar, A.,Panduro, A.,etc.Treatment with anti-transforming growth factor antibodies influences an altered pattern of cytokines gene expression in injured rat liver[J].1998,1442(1).