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Prolongation of survival peirod of islets by ICCs encapsulated by semipermeable membrane xenografted in brain
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.0254-1785.2003.05.123
- Key Word:
- 胰岛;移植,异种;脑;大鼠;狗
Abstract： 目的研究生物半透膜包裹胰岛样细胞团(ICCs)的免疫隔离效果.方法分离培养SD大鼠的ICCs,用生物半透膜包裹,体外观察半透膜的通透性,并将包囊后的ICCs植入犬大脑实质内,术后1个月和2个月以光镜和电镜观察移植物和周围脑组织的病理改变,免疫组化染色鉴定移植物内的胰岛B细胞.结果半透膜包囊在整个体外实验过程中无破损,淋巴细胞及其它血细胞成份不能透过半透膜,而葡萄糖和胰岛素能迅速弥散透过半透膜囊.移植后2个月,移植物内无多形核细胞和淋巴细胞的团状浸润,免疫组化染色证明移植物内有胰岛B细胞存在,周围的脑组织仅有轻度的胶质细胞增生和水肿.结论半透膜包裹ICCs结合免疫特赦部位移植的方法对克服异种胰岛移植的免疫排斥反应有一定的作用.
- [1]万赤丹,陈实,陈刚,等.猪胰岛在糖尿病大鼠不同部位异种移植的实验研究[J].1998,(4).
- [2]G C, Weir,S, Bonner-Weir.Scientific and political impediments to successful islet transplantation.[J].1997,46.
- [3]Soderlund J,Groth CG,Biberfeld P,etc.Fetal porcine islet-like cell clusters transplanted to cynomolgus monkeys: an immunohistochemical study.[J].1999,67(6).
- [4]A K, Ommaya,I, Atwater,B A, Coonrod,etc.Novel transplantation approach: islet cell grafts in cerebral spinal fluid shunts in dogs.[J].1994,26.
- [5]信照亮,吕健,段国荣,等.局部应用地塞米松对创伤性脑水肿及血脑屏障通透性的影响[J].1997,(6).