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The role of mycophenolate acid on HBV replication in vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF ORGAN TRANSPLANTATION
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.0254-1785.2003.05.122
- Key Word:
- 霉酚酸;肝炎病毒,乙型;抗原;细胞株
Abstract： 目的探讨霉酚酸(MPA)在体外对乙型肝炎病毒(HBV)基因复制、病毒蛋白合成的影响.方法将不同浓度的MPA(1～50 mg/L)作用于 2.2.15细胞,12 d后用酶联免疫吸附试验检测培养上清液中乙型肝炎表面抗原(HBsAg)和e抗原(HBeAg),狭缝印迹杂交法定量分析培养细胞内的HBV DNA.结果霉酚酸对HBV的复制起轻微的抑制作用,随着浓度的增加,抑制作用逐渐增强;50 mg/L的MPA对HBsAg、HBeAg的抑制率分别为 24.05 %和 30.20 %,HBV DNA 的表达量仅为对照组的 49 %.结论霉酚酸具有抑制 2.2.15细胞分泌HBsAg、HBeAg和复制HBV DNA的作用,并呈剂量依赖性.
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