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Inhibitory effects of connective tissue growth factor siRNA in high glucose induced connective tissue growth factor and collagen synthesis in rat mesangial cells
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- Author:
- No author available
- Journal Title:
- CHINA MEDICINE
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1673-4777.2010.02.013
- Key Word:
- 糖尿病性肾病;RNA干涉;结缔组织生长因子;大鼠系膜细胞;Diabetic nephropathy;RNA interference;Connective tissue growth factor;Rat mesangial cell
Abstract： Objective To investigate the inhibitory effects of siRNA targeting CTGF on the expression of CTGF and collagen type Ⅰ in cultured rat mesangial cell (RMC) with high glucose. Methods SiRNA targeting CTGF was designed, synthesized and transfected into RMC cultured with high glucose. Expression of CTGF and col-lagen type Ⅰ was examined by reverse transcription polymerase chain reaction(RT-PCR) and western blot. Results After siRNA was transfected, expression of CTGF and collagen type Ⅰ decreased significantly compared with that in RMC cultured with only high glucose. Conclusion The siRNA targeting CTGF could inhibit synthesis of CTGF and collagen type Ⅰ in RMC cultured with high glucose.
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