Abstract： Objective:To reconstruct zygomatico-orbtial and maxillary bone defects using three-dimensional (3D) printing technology, so as to provide the basis for complicated maxillofacial bone defects.Methods:Five patients diagnosed with in zygomatico-orbtial and maxillary neoplasm in Department of Oral and Maxillofacial Surgery, General Hospital of Chinese PLA, who need bone defect reconstruction after surgery. Two different customized prosthesis were fabricated by computer aided design and 3D printing techonology, and the length of orbital floor extension in the customized prosthesis were different: Design 1, 9-10 mm orbital floor extension; Design 2, 10-15 mm orbital floor extension. The clinical outcome were evaluated during operation and matching condition of two different designed prosthesis were carried out after scanning for analysis.Results:The results indicated that the deviation value were 2-3 mm located at fixed structure during clinical evaluaton, and the deviation value were about 1 mm after prosthesis scanning. Finally, prothesis of Design 1 were applied for clinical use, and satisfactory reconstruction contour was achieved in all patients.Conclusions:The results suggest that zygomatico-orbtial and maxillary bone defects reconstruction can be conducted with satisfactory effect using 3D printing technology, and design and fabrication factors should be taken into consideration in complicated structure design with multi-protuberance.