Abstract： Objective To explore a digital negative molds technique based on three-dimensional (3D) printing to assist in the manufacture of maxillofacial prostheses,and to improve the deficiency of the current clinical treatment.Methods Seventeen patients with maxillofacial defects (including nasal defects,orbital defects,cheek defects,auricle defect) were scanned by means of facial optical scanning and computer tomography (CT).The 3D models were then reconstructed and global registration was made to merge the reconstructed models into a new digital model for 3D design.The 3D design of the prostheses was implemented in software.The mechanical connection structure was designed by forward engineering technology for 3 patients with intra-oral defects in maxilla who needed to make removable partial dentures,so that the silicone prostheses and removable partial denture could be combined.The removable partial dentures were made by conventional method and connected with the prostheses.According to the 3D data of the prostheses,the digital negative molds were designed,and the 3D printing technology was used to finish the processing of the resin molds.Silicone for prostheses were filled and cured in the resin molds to fabricate the clinical restorations for the patients.The margin adaptation and retention of the prostheses was detected.Results Twenty patients with varying degrees of maxillofacial defects were rehabilitated using the courses developed in the study.All patients reported no pain or discomfort during the treatment;and they were satisfied with the final prostheses of the shape,color,retention,stability,etc.Eighteen of the prostheses showed good marginal adaptation,and sixteen of the prostheses showed good retention effect.Conclusions The digital negative molds technique used in this study could greatly reduce the intensity of manual operation and provided a good therapeutic effect for patients with maxillofacial defects.