Abstract： Objective To evaluate the dentin and enamel bonding strength and resin-dentin interfaces of four resin cements. Methods Twenty-four sound freshly extracted molars were sectioned with low-speed saw under running water. Two mesial-distal enamel disc and two buccal-lingual dentin discs were sectioned from each molar. Four resin cements( A group: PanaviaTM F; B group: RelyXTM ARC; C group:RelyXTM Unicem; D group: ClearfilTM SA Cement) were applied to the surface of these enamel and dentin discs according to manufacturers' guidance. The bonded specimen were prepared for shear bond strength test and sectioned occluso-gingivally into two slabs for scanning electron microscopy(SEM) observation. Results The bonding strength between resin and enamel in B group[ (29. 38 ±6. 28) MPa] was higher than that in D group[ (27.25 ± 7.03) MPa], and both of them were significantly higher than those in A [ ( 22.92 ±7. 13) MPa] and C group [ ( 10.92 ± 3.41 ) MPa] ( P ＜ 0.05 ). The highest dentin bonding strength was shown in B group [ ( 27.28 ± 6. 79 ) MPa ], followed by A [ ( 14. 23 ± 6. 39 ) MPa ], D [ ( 10.09 ±3.26) MPa] and C group[ (6. 43 ± 1.60) MPa, P ＜ 0.05 ]. Conclusions The four resin cements have different enamel or dentin bonding properties.