Abstract： Objective:To provide references on evaluating autonomic nervous stability training effect of military flying personnel by studying the evaluation index of heart rate variability (HRV).Methods:The ECG signals of 98 military flying personnel before and after three-stage autonomic nervous stability training were collected, and the ratio of 0.1Hz power (R) of HRV was calculated. The R value before and after personal training was marked as R 1 and R 2 respectively, and the D value was used to represent the difference value between R 2 and R 1. The feasibility of using R 2 and D to evaluate the training effect was analyzed to formulate evaluation standards. In order to more intuitively display an individual′s autonomic nervous stability training level, the Z value was used to convert the original score of R 2 and D, and the evaluation criteria were formulated. Results:The R value after autonomic nervous stability training (R 2=0.473±0.248) was significantly higher than that before (R 1=0.197±0.092, P<0.01), and the individuals with lower R value before training have more obvious growth trend of R value after training. Conclusions:"0.1Hz index" is suitable to be the evaluation index of autonomic nervous stability training effect. The evaluation of autonomic nervous stability training effect of flying personnel should comprehensively consider the grade evaluation results of R 2 and D.