Abstract： Objective To study the gradual-onset rate (GOR)mode on human centrifuge G-tolerance determination and the applications in pilot selection and training. Methods Twenty-five pilots were undertaken the G-tolerance examination under centrifuge GOR operating mode and completed high G centrifuge training at +8.0 Gz level for 10 s.The pilots were divided into excellent group (n=1 3),good group (n=7 )and mediocre group (n=5 )by the high G training result.The pilots, G-tolerance under GOR mode and the anti-G effect of performing HP maneuver were analyzed. Results Under GOR mode pilots, strained tolerance (GOR2 )was significantly higher than their relaxed tolerance (GOR1 )(t=1 4.420,P=0.000 ).The differences on GOR1 tolerance and HP maneuver anti-G effect were not significant among different groups (F=2.593,0.1 76,P>0.05).The GOR2 tolerance of excellent group was significantly higher than that of other 2 groups (P<0.05). There was no significant difference on GOR2 tolerance among groups (P>0.05). Conclusions The G-tolerance and the effect of performing anti-G straining maneuver could be precisely and conveniently evaluated with GOR mode.It is necessary to establish the G-tolerance examination method and assessment criterion under centrifuge GOR mode for the G tolerance determination and training of high performance fighter aircraft pilot.