Abstract： Objective To observe the effects of combined shipboard chronic fatigue factors induced by prolonged deployment at sea on auditory function in rats.Methods Thirty-six male SD rats were randomly divided into 3 groups:the blank control group,the sleep deprivation control group and the combined factors group.The chronic fatigue animal model was developed by using 3 combined factors of noise exposure,sleep deprivation and simulated motion sickness.Deformation product otoacoustic emission (DPOAE) amplitudes were recorded with the DPOAE smart system in rats before and after experiment.Blood samples were collected,and detection of such serum biochemical indices as corticol (CORT),malondialdehyde (MDA) and super oxide dismutase (SOD) were made.Results The chronic fatigue animal model induced by combined main shipboard factors was established in rats.Changes in the amplitudes of DPOAE for the animals of the combined factors group were not statistically significant (P ＜ 0.05),when they were compared with those of the blank control group.Serum SOD activity decreased for the animals of the combined factors group,when compared with that of the blank control group(P ＜0.05).The contents of MDA and CORT tended to increase,but no statistical differences could be noted (P ＞ 0.05).Conclusions Influenced by combined factors of chronic fatigue from prolonged deployment at sea,the rats were in a state of chronic stress,nevertheless,no obvious auditory impairment could be detected in rats.