Damage to peripheral nerves induced by Campylobacter jejuni exotoxin
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PEDIATRICS
- Issue:
- 12
- DOI:
- 10.3760/cma.j.issn.0578-1310.2003.12.118
- Key Word:
- 周围神经系统疾病,弯曲杆菌,空肠;细菌毒素类
Abstract： 目的探讨空肠弯曲菌毒素致周围神经损伤的作用机理.方法 (1)将PEN 19-CJ毒素(CJT)免疫后的Wistar大鼠血清,以及体外激活的该鼠PBMC上清液,分别注入健康大鼠神经外膜下及静脉内.(2)取大鼠坐骨神经进行单纤维分离,甲苯胺蓝光镜和电镜超微组织病理学检测.(3)用SABC及FITC-免疫荧光法对与CJT共同作用后的正常大鼠和人坐骨神经,以及CJT致病变神经组织标本进行免疫酶组织化学和免疫荧光检测,并以核酸杂交技术检测其TNF-α mRNA表达.结果 (1)CJT经外膜下及静脉注射均可诱发坐骨神经明显轴索变性及髓鞘脱失病理学改变.外膜下注射组原纤维病变率为76.8%(2 763/3 600),对照组仅为9.6% (230/2 400)(P＜0.01).静脉注射后病变原纤维发生率于第3天达高峰,为19.5%(390/2 000),持续至第14天时病变仍为15.5%(310/2 000),而对照组始终无病变发生(P＜0.01).CJT免疫血清及其PBMC上清液外膜下注射原纤维病变率与对照组无明显差异(P>0.05).(2)CJT与正常大鼠和人坐骨神经共同孵育后,以及CJT致周围神经病变组织免疫化学检测显示不同程度的CJT结合阳性反应.(3)CJT静脉注射后,在周围神经已出现明显病变时,其他脏器未见明显组织学异常.结论 (1)CJT对周围神经具有直接毒性损伤作用.在神经组织已有明显病变情况下,脑、肝、肾等脏器未见明显改变,提示CJT对周围神经组织损伤的相对特异性.(2)CJ诱发的吉兰-巴雷综合征中,CJT本身无明显免疫学致病力,可能通过其他途径增强了LPS对周围神经的免疫损伤作用.
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