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Gastric epithelial cell proliferation, apoptosis and Fas gene in children with Helicobacter pylori associated chronic gastritis
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PEDIATRICS
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.0578-1310.2002.03.108
- Key Word:
- 螺杆菌,幽门;螺杆菌感染;胃炎;胃粘膜;脱噬作用;抗原;CD95
Abstract： 目的研究幽门螺杆菌(HP)感染儿童胃粘膜上皮细胞的增殖、凋亡及凋亡相关基因Fas的变化,对幽门螺杆菌的致病机制进行探讨.方法采用免疫组织化学和Tunel法检测17例HP阳性小儿慢性胃炎和19例HP阴性的小儿慢性胃炎胃粘膜上皮细胞增殖、凋亡和凋亡相关基因Fas的变化.结果 HP阳性组的胃粘膜上皮细胞增殖指数为(9.02±3.82)%,凋亡指数为(8.46±1.82)%,Fas指数为(31.29±12)%, HP阴性组胃粘膜上皮细胞增殖指数为(3.41±1.33)%,凋亡指数为(2.73±0.85)%,Fas指数为(11.68±9)%.三者在HP阳性组与HP阴性组之间差异有显著意义,与年龄性别无关;胃粘膜上皮细胞增殖指数在轻度胃炎为(3.94±3.51)% ,中度胃炎为(6.53±4)%,重度胃炎为(13.65±7)%,三者间差异有显著意义;凋亡指数在轻度胃炎为(3.08±1.05)%,中度胃炎为(5.82±3)%,重度胃炎为(17.81±2.63)%,三者间亦差异有显著意义.细胞的增殖和凋亡在炎症程度间差异有显著意义.结论 HP促进胃粘膜上皮细胞的增殖和凋亡;Fas的表达在HP阳性组明显增加 ,提示Fas的过度表达可能是小儿HP相关性胃炎胃上皮细胞凋亡增加的原因之一.
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