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Expression of E-selectin in cerebrovascular endothelium in a brain inflammation model
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PEDIATRICS
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.0578-1310.2002.01.109
- Key Word:
- 中枢神经系统感染;E选择素;地塞米松;内毒素类;内皮,血管
Abstract： 目的研究中枢神经系统感染时血管内皮细胞E选择素的表达,以及地塞米松对其抑制作用.方法将54只小鼠随机分成侧脑室注射内毒素(20 μg/20 μl)组、腹腔注射地塞米松 [1 mg/(kg*12 h)]+侧脑室注射内毒素(20 μg/20 μl)组、生理盐水组以及空白对照组.免疫组织化学法观察脑血管内皮细胞E选择素的表达,HE染色观察脑组织炎症反应.结果空白对照组有少量E选择素表达[(13.7±2.7)%].生理盐水组[(13.5±5.6)%]与空白对照组E选择素表达比较差异无显著性.内毒素组注射后E选择素表达在第1天 [(31.8± 10.5)%]、第2天 [(26.8±9.6)%] 明显增多,与生理盐水组比较差异有显著性(P<0.01或 <0.05),注射后第3天 [(15.7±9.9)%]恢复正常(P＞0.05);注射后3 d内E选择素表达呈进行性下降(F=4.08,P＜0.05).地塞米松+内毒素组于注射后第1天和第2天E选择素表达与内毒素组比较明显受到抑制,分别为(13.0±12.4)%、(8.8±6.0)%(P略>0.05,P＜0.05),与生理盐水组比较差异无显著性;同时脑组织炎症反应受到明显抑制.结论 中枢神经系统感染时脑血管内皮细胞E选择素的表达增加;地塞米松可通过抑制脑血管内皮细胞表面E选择素的表达来抑制炎症反应.
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