Abstract： Objective:To investigate the clinical and immunological characteristics of idiopathic inflammatory myopathies(IIMs)patients with positive anti-Ro-52 antibody.Methods:This study was a retrospective study.A total of 110 patients with IIMs admitted to the department of Rheumatology and Immunology, General Hospital of Northern Theater Command from January 2016 to December 2021 were selected, including 37 males and 73 females, aged(52.14±15.16)years old, ranging from 21 to 83 years old.Clinical data and laboratory indicators were collected.Results:Among 110 IIMs patients, 28 were positive for anti-Ro-52 antibody and 82 were negative for anti-Ro-52 antibody, all patients were divided into positive group and negative group according to the positive status of anti-Ro-52 antibody.Positive group of patients with female[85.7%(24/28)]much higher than negative group[59.8%(49/82)], pulmonary interstitial disease prevalence rate[64.3%(18/28)]was higher than negative group[30.5%(25/82)].Mechanic hand[21.4%(6/28)]was higher than negative group[6.1%(5/82)], V type rash or anterior portion shawl rate[10.7%(3/28)]was lower than the negative group[31.7%(26/82)].The proportion of short breath after activity[28.6%(8/28)]was higher than that of negative group[8.5%(7/82)], and the differences were statistically significant( P<0.05).The levels of hemoglobin and albumin in the positive group were lower than those in the negative group, the positive rate of antinuclear antibodies, the positive rate of anti-aminoacyl-tRNA synthetase antibodies(ARS), the levels of creatine kinase, 1actate dehydrogenase, alanine aminotransferase, aspartate amino transferase, immunoglobulin G, immunoglobulin A and immunoglobulin M were higher than those in the negative group, and the differences were statistically significant( P<0.05).In the positive group, anti-MDA5 antibody was positive in 3 patients, anti-HMGCR antibody was positive in 1 patient, and ARS was positive in 15 patients.In the negative group, anti-MDA5 antibody was positive in 2 patients, anti-TIF-1γ antibody was positive in 1 patient, anti-NXP 2 antibody was positive in 1 patient, anti-M1-2 antibody was positive in 1 patient, anti-SRP antibody was positive in 2 patients, and ARS 6 patients. Conclusions:Anti-Ro-52 antibody can lead to a series of atypical clinical manifestations of IIMs, most of which are accompanied by positive antinucleic antibody.It is necessary to pay attention to the possibility of ARS syndrome in patients with positive anti-Ro-52 antibody, and early screening of lung interstitial lesions, myoenzyme levels and myositis specific antibody profiles.