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Expression of MMP-9 gene in MCAO rats:a DTI study
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Biomedical Engineering
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1674-1927.2018.02.004
- Key Word:
- 基质金属蛋白酶-9;脑梗死;扩散张量成像
Abstract： 目的 通过对大鼠大脑中动脉栓塞(MCAO)模型脑缺血区组织的DTI成像评价,探讨缺血区的ADC值及FA值与MMP-9基因表达的相关性.方法 选择56只SD大鼠,将其中16只SD大鼠,随机分为两组(每组8只):A组(假手术组)和B组(MCAO组),于MCAO后4、8、12、18、24 h时间点,计算病灶侧的ADC值和FA值.另40只SD大鼠按上述时间点进行Western blotting和免疫组化(分别20只),测定缺血侧脑组织MMP-9蛋白表达.结果 MCAO模型组大鼠缺血区ADC值、FA值均低于假手术组,12 h降至最低,随后略有升高,两组间的ADC值、FA值在4、8、12、18、24 h时间点差异均具有统计学意义(P<0.05);MCAO模型组大鼠缺血后,不同时间点缺血区的MMP-9蛋白表达增加,且在4、8、12、18、24 h时间点差异均具有统计学意义(P<0.05).MCAO组大鼠缺血区的ADC值及FA值与MMP-9蛋白表达存在负相关.结论 磁共振DTI序列可动态监测缺血性脑梗死大鼠缺血区的改变,脑梗死区脑组织MMP-9表达参与大鼠脑梗死周围水肿的形成以及神经功能损伤的发生.
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