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lncRNA as a competing endogenous RNA regulates development of psoriasis:a mechanistic study
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Biomedical Engineering
- Issue:
- 2
- DOI:
- 10.3760/cma.j.issn.1674-1927.2018.02.001
- Key Word:
- 银屑病;lncRNA;microRNA;ceRNA;随机游走
Abstract： 目的 探讨长链非编码RNA(lncRNA)在银屑病中作为竞争性内源RNA(ceRNA)调控微小RNA(miRNA)和基因表达的临床与病理意义.方法 下载银屑病患者正常组织、非皮损组织、皮损组织3类60个样本的高通量测序数据(GSE63980),通过生物信息的研究方法构建了银屑病不同类型样本之间差异lncRNA-mRNA共表达网络.通过StarBase数据库下载miRNA、lncRNA和mRNA的靶向关系对,通过计算二者的皮尔森相关系数(r≥0.7,P<0.05)和共享miRNA靶点,构建了银屑病竞争性内源RNA(ceRNA)网络.利用文献证实的银屑病相关的miRNA、lncRNA、mRNA作为种子节点计算随机游走得分,筛选出银屑病相关的lncRNA,并对部分结果开展了GO(gene ontology)功能分析和KEGG通路分析,通过文献检索验证了部分lncRNA具有潜在的ceRNA关系.结果 识别了正常和皮损组织中显著差异的677个mRNA,356个lncRNA;正常和非皮损组织中显著差异的240个mRNA,77个lncRNA;皮损和非皮损组织中显著差异的612个mRNA,209个lncRNA.在共表达网络中的mRNA主要参与的GO通路有G蛋白偶联受体活性、DNA绑定、氮化合物代谢等,KEGG信号通路主要有嗅觉传导、味觉传导等.在ceRNA网络中的mRNA主要参与的GO通路有跨膜受体活性、信号受体活性等,KEGG信号通路主要有系统性红斑狼疮,自身免疫疾等.结论 GAS5与HOTAIR等多个lncRNA在银屑病不同样本中通过与基因竞争miRNA形成潜在的ceRNA关系,有望成为银屑病诊断,治疗的靶标.
- [1]徐可佳.银屑病全基因组关联的易感基因和信号通路的研究进展[J].2016,(26).
- [2]郭小伟.银屑病的治疗进展[J].2016,(7).
- [3]饶朗,陈永锋.miRNA 与银屑病相关研究新进展[J].2016,(12).
- [4]梅沉成,张云璧,霍春波,等.miRNA调控银屑病角质形成细胞增殖机制的研究进展[J].2016,(11).
- [5]王靖,顾艳丽,张新萍,等.非编码RNA在银屑病中的研究进展[J].2017,(1).
- [6]孙敏,田中伟.IL-33/ST2/NF-κB在银屑病中作用的研究进展[J].2016,(11).
- [7]Pickard,M.R.,Mourtada-Maarabouni,M.,Williams,G.T..Long non-coding RNA GAS5 regulates apoptosis in prostate cancer cell lines[J].2013,1832(10).
- [8]Zhang Haihong,Han Hua,Ye Kaishan,etc.Long Noncoding RNA GAS5 Suppresses Cell Growth and Epithelial-Mesenchymal Transition in Osteosarcoma by Regulating the miR-221/ARHI Pathway[J].2017,118(12).
- [9]Tsoi, Lam C.,Iyer, Matthew K.,Stuart, Philip E.,etc.Analysis of long non-coding RNAs highlights tissue-specific expression patterns and epigenetic profiles in normal and psoriatic skin[J].2015,16(1).
- [10]Salmena, L.,Poliseno, L.,Tay, Y.,etc.A ceRNA hypothesis: The rosetta stone of a hidden RNA language?[J].2011,146(3).
- [11]Edward A. Codling,Michael J. Plank,Simon Benhamou.Random walk models in biology[J].2008,5(25).
- [12]Fu, Dandan,Yu, Wenfa,Li, Min,etc.MicroRNA-138 regulates the balance of Th1/Th2 via targeting RUNX3 in psoriasis[J].2015,166(1).
- [13]Jun-Hao, Li,Shun, Liu,Hui, Zhou,etc.starBase v2.0: decoding miRNA-ceRNA, miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data.[J].2014,42.
- [14]Xie SL,Ma J,Wang GY,etc.Large Intervening Non-coding RNA HOTAIR is Associated with Hepatocellular Carcinoma Progression.[J].2011,39(6).
- [15]Joel R Neilson,Margaret S Ebert,Phillip A Sharp.MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells[J].2007,4(9).
- [16]Hawkes, Jason E.,Nguyen, Giang Huong,Fujita, Mayumi,etc.microRNAs in Psoriasis[J].2016,136(2).
- [17]Batista, P.J.,Chang, H.Y..Long noncoding RNAs: Cellular address codes in development and disease[J].2013,152(6).
- [18]Yin, Dandan,He, Xuezhi,Zhang, Erbao,etc.Long noncoding RNA GAS5 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer[J].2014,31(11).
- [19]Lam C, Tsoi,Sarah L, Spain,Jo, Knight,etc.Identification of 15 new psoriasis susceptibility loci highlights the role of innate immunity.[J].2012,44.
- [20]Skov L,Lovendorf MB,Kaczkowski B,etc.MicroRNAs and potential target interactions in psoriasis.[J].2010,58(3).
- [21]Sha, Yan,Zhenyao, Xu,Fangzhou, Lou,etc.NF-κB-induced microRNA-31 promotes epidermal hyperplasia by repressing protein phosphatase 6 in psoriasis.[J].2015,6.
- [22]Gui, Zhao,Zhenyi, Su,Dan, Song,etc.The long noncoding RNA MALAT1 regulates the lipopolysaccharide-induced inflammatory response through its interaction with NF-κB.[J].2016.
- [23]Zhang,Z.,Zhu,Z.,Watabe,K.,etc.Negative regulation of lncRNA GAS5 by miR-21[J].2013,20(11).
- [24].Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis[J].2010,463(Apr.15 TN.7291).
- [25]Frank,J.,Johnson,G.,Jutooru,I.,etc.HOTAIR is a negative prognostic factor and exhibits pro-oncogenic activity in pancreatic cancer[J].2013,32(13).
- [26]李萌.肝癌中miR-221对长链非编码RNA HOTAIR转录后水平调控机制的研究[D].2014.