Abstract： Objective To develop a novel device for in vitro fixation of skin and measurement of transcutaneous electrical resistance.Methods This device was consisted of in vitro skin fixation apparatus and an instrument for measuring electrical resistance.The fixation apparatus was filled with electrolyte,thus allowing for the formation of a conductivity cell between the inner and outer surface of skin,which measured the transcutaneous electrical resistance via the inner and outer electrodes.Following fixation to the apparatus,eight normal skin tissues derived from a Wistar rat received,the measurement of transcutaneous resistance at 18 time points,which was repeated for three times.Results The measured data were within the normal range and three repeated experiments showed consistency in the skin resistance curves suggesting that this device was capable of maintaining the in vitro stability of skin barrier function within 24 hours.Conclusion The newly developed device satisfies the requirements of alternative methods for in vitro skin irritation/corrosion tests and can be applied for in vitro drug release,transcutaneous absorption tests and the measurement of transcutaneous electrical resistance.