Abstract： Objective To study the effects of wild-type hypoxia-inducible factor-1α (HIF-1α) on proliferation,migration and tube formation in vitro in human lung microvascular endothelial cells (HMVEC-Ls).Methods The HMVEC-Ls were divided into normal control group,physical hypoxia group and HIF-1α transfection group.The MTS,wound-healing migration,and Matrigel assays were employed to determine the proliferation,migration and tube formation,respectively.The protein expressions of HIF-1α and VEGF were analyzed by Western blotting.Results Compared with normal control and physical hypoxia groups,transfection of HMVEC-L with wild-type HIF-1αt at day 3 resulted in pronounced HMVEC-L proliferation (P＜0.05),a significantly augmented migration rate of the genome [(1.09±0.25)％ and (8.65±1.03)％ vs (17.12±1.97)％,P＜0.05] and substantially increased length of tube [(0.34±0.07) and (0.90±0.16) vs (1.76±0.09) mm/field,P＜0.05].Western blotting assay evidenced significantly augmented HIF-1αt and VEGF expressions in physical hypoxia and wild-type HIF-1α groups (all P＜0.05),particularly the latter.Conclusion Wild-type HIF-1α promotes angiogenesis by facilitating HMVEC-L proliferation,migration and tube formation in vitro and is expected to be a promising target for therapeutic angiogenesis.