Abstract： Objective To explore the effects of Qing-Yi Decoction (QYD) on gene expression profile in rats with sodium taurocholate-induced acute necrotizing pancreatitis(ANP).Methods Sixty SD rats were randomly assigned to Sham operation group(group SO,n=20),ANP group(n=20) and QYD group (n=20).The ANP model was established by pancreatic duct retrograde injection with 4％ sodium taurocholate.SO group was treated with normal saline and QYD group received intragastric injection of QYD (0.75 ml/100 g) for thrice.The survival rates and changes in serum amylase and C-reactive protein (CRP)were determined.HE staining was emnployed for assessment of pathological changes in the pancreas and lung tissues.Measurement of the altered pancreatic RNA expression was conducted by using Illumina wholegenome expression biochips.Changes in particular genes,namely,Hspa8 and Hspb1,were verified via quantitative reverse transcriptase polymerase chain reaction(QRT-PCR).Results Compared with group ANP,a higher survival rate (80％ vs 65％,P=0.031) and Schmidt pancreas pathological scores yet reducedserum amylase[(5789±798) U/L vs (7256±1221) U/L,P=0.001] and CRP[(78.6±18.5) mg/L vs (126.4±24.3) mg/L,P=0.012] were noted in group QYD.Of the 575 differential genes,when compared with group ANP,group QYD yielded 92 up-regulated and 483 down-regulated genes.The gene ontology was employed to analyze the negative modulation of transcription regulator,oxidoreductase and enzyme inhibitor activity.Effects of QYD on ANP were mainly linked to KEGG metabolic pathways that involved mitogen-activated protein kinase (MAPK) and NOD receptor-like signaling pathway,cell cycle,metabolic pathways and oxidoreductase activity.Changes of Hspa8 and Hspb1 mRNA in microarray were verified by QRT-PCR.Conclusion QYD is effective for the treatment of experimental ANP as a consequence of altered MAPK and NOD-like receptor signaling pathways,cell cycle,metabolic pathways and oxidoreductase activity.