Abstract： Objective To investigate the effects of different shear stress on expression of lectinlike oxidized low-density lipoprotein receptor-1 (LOX-1 ) on endothelial cell surface. Methods The human umbilical vein endothelial cells (HUVECs) were cultured in vitro and implanted on microscopic slides which were then put into a flow chamber system and treated with different shear stress. According to different shear stress, HUVECs were allocated into control group (0 dyne/cm2, n=5) and experimental groups including low shear stress group (4.2 dyne/cm2, n=5), moderate shear stress group (8.4 dyne/cm2, n=5) and high shear stress group ( 15.0 dyne/cm2, n=5). The mRNA expression of LOX- 1 of the endothelial cells was detected in all groups with RT-PCR at 1 h, 2 h, 4 h and 8 h after loading of shear stress. Results The expression of LOX- 1 increased over time under low shear stress (4.2 dyne/cm2). Compared with the control group, the expressions of LOX- 1 in endothelial cells were declined at 2 h, 4 h and 8 h after treatment with moderate shear stress (8.4 dyne/cm2) [2 h: 0.2346 ± 0.0397 vs 0.2948 ± 0.0128; 4 h: 0.1747 ± 0.0585 vs 0.2985 ±0.0204; 8 h: 0.0256±0.0067 vs 0.2968±0.0175, all P＜0.05]. However, the expression of LOX- 1 maintained a very low level under high shear stress (15.0 dyne/cm2). Conclusion Different shear stress may have varied effects on the expression of LOX-1 in endothelial cells.