Transcriptional study of outer membrane protein in abnormal regulation about biofilm
- Author:
- No author available
- Journal Title:
- International Journal of Respiration
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1673-436X.2017.11.005
- Key Word:
- 多药耐药鲍曼不动杆菌;生物被膜;外膜蛋白;转录组学研究;Multidrug resistant acinetobacter baumannii;Biofilm;Outer membrane protein;Transcriptional study
Abstract： Objective To study the role of outer membrane protein in the formation of multidrug resistant Acinetobacter baumannii biofilm.Methods The multidrug resistant strains of Acinetobacter baumannii in our hospital were selected.The same strain was studied in different growth states,the whole genome of the two samples was compared.The gene function was analyzed by high-throughput sequencing.Statistical differences in gene expression between two samples were studied.Results The gene expression of the same strain in two different states was indeed different.Among the differentially expressed genes,106 genes were up-regulated and 92 genes were down-regulated.Among them,the outer membrane protein related genes in the biofilm state were significantly expressed.Conclusions The biofilm was a unique form.In the biofilm state,the different genes focus on the expression of the outer membrane proteins and the regulation of intracellular pathways.Outer membrane protein,which is one of most abundant proteins in the biofilm formation,plays an important role in the process of biofilm formation and maturation.
- [1]Seifert H,Peleg AY,Paterson DL.Acinetobacter baumannii: emergence of a successful pathogen.[J].2008,21(3).
- [2]Praveen K, Sahu,Pavithra S, Iyer,Amrita M, Oak,etc.Characterization of eDNA from the clinical strain Acinetobacter baumannii AIIMS 7 and its role in biofilm formation.[J].2012,2012.
- [3]Ming-Feng Lin,Chung-Yu Lan.Antimicrobial resistance in Acinetobacter baumannii: From bench to bedside[J].2014,(12).
- [4]Cabral, M.P.,Soares, N.C.,Aranda, J.,etc.Proteomic and functional analyses reveal a unique lifestyle for acinetobacter baumannii biofilms and a key role for histidine metabolism[J].2011,10(8).
- [5]Carlos A, García,Eliana S, Alcaraz,Mirta A, Franco,etc.Iron is a signal for Stenotrophomonas maltophilia biofilm formation, oxidative stress response, OMPs expression, and virulence.[J].2015,6.
- [6]Espinal,P.,Vila,J.,Martí,S..Effect of biofilm formation on the survival of Acinetobacter baumannii on dry surfaces[J].2012,80(1).
- [7]Harper,M.,Adler,B.,Boyce,J.D.,etc.Lipopolysaccharide-deficient acinetobacter baumannii shows altered signaling through host toll-like receptors and increased susceptibility to the host antimicrobial peptide LL-37[J].2013,81(3).
- [8]McConoughey, Stephen J.,Howlin, Rob,Granger, Jeff F.,etc.Biofilms in periprosthetic orthopedic infections[J].2014,9(8).
- [9]Gaddy JA,Tomaras AP,Actis LA.The Acinetobacter baumannii 19606 OmpA protein plays a role in biofilm formation on abiotic surfaces and in the interaction of this pathogen with eukaryotic cells.[J].2009,77(8).
- [10]Cerqueira,G.M.,Kostoulias,X.,Khoo,C.,etc.A global virulence regulator in acinetobacter baumannii and its control of the phenylacetic acid catabolic pathway[J].2014,210(1).
- [11]Wang, Yun,Wilks, Jessica C.,Danhorn, Thomas,etc.Phenazine-1-Carboxylic Acid Promotes Bacterial Biofilm Development via Ferrous Iron Acquisition[J].2011,193(14).
- [12].Bactericidal activity in filtrated supernatant of Streptococcus sanguinis against multidrug-resistant Pseudomonas aeruginosa.[J].2009,219(2).