Expression of several molecules correlated with vitamin D in bronchus of asthmatic mouse
- Author:
- No author available
- Journal Title:
- INTERNATIONAL JOURNAL OF RESPIRATION
- Issue:
- 11
- DOI:
- 10.3760/cma.j.issn.1673-436X.2010.011.006
- Key Word:
- 支气管哮喘;支气管组织;维生素D;Bronchial asthma;Bronchus;Vitamin D
Abstract： Objective To investigate the expression of several molecules correlated with vitamin D and the effect of dexamethasone on them in bronchus of asthmatic mouse. Methods Eighteen BALB/c mice were divided equally into a control,asthmatic and treated groups. The asthmatic mice were sensitized with ovalbumin. The treated mice were injected with dexamethasone via intraperitoneal. After the challenge, the bronehialalveolar lavage fluid(BALF) was collected for examining the cell number and the level of IL-4, and the bronchus tissue was taken to evaluate the airway inflammation. The expressions of VDUP1 ,VDR and CYP27B1mRNA were measured with real-time RT-PCR. Results The levels of VDUP1mRNA, VDR mRNA and CYP27 B1 mRNA in asthmatic bronchus were significantly higher(2.74 ± 0.99,7.06±4.05,3.40 ± 2.16, respectively) than those in control ( 1.01 ± 0.18, 1.28 ± 0. 76, 1.45 ± 1.39,respectively, P ＜0. 05) and those in treated groups (0.94±0. 34,0. 76±0.18,0. 27±0.17,respectively,P ＜0.01). Conclusions Several molecules correlated with vitamin D may be involved in the physiopathologic process in asthma.
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