Relationship between p21ras and angiogenesis in chronic pancreatitis,pancreatic cancer and its clinical significance
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF PANCREATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/cma.j.issn.1674-1935.2001.01.007
- Key Word:
- 血管形成;慢性胰腺炎;胰腺癌;p21ras;CD34
Abstract： 目的探讨慢性胰腺炎(chronic pancreatitis,CP)、胰腺癌(pancreatic adenocarcinoma,PAC)组织中血管生成与ras基因产物p21蛋白表达的关系及在慢性胰腺炎、胰腺癌演化过程中所起的重要作用.方法应用免疫组化Envision方法检测7例正常胰腺、24例慢性胰腺炎、24例胰腺癌组织中CD34、p21ras的表达.对CD34阳性血管进行微血管密度(microvessel density,MVD)计数.结果CD34抗体染色大部分毛细微血管和单个内皮细胞.最高微血管染色区域(hotspot)几乎总是在肿瘤与周围组织交界的浸润缘和慢性炎症组织,特别是纤维化组织.对照组正常胰腺组织除腺泡的微血管血供外,叶间结缔组织很少能观察到新生血管.p21ras阳性物可见于增生性胰腺导管上皮细胞及导管腺癌细胞,正常胰腺无表达,癌组织(47.2±9.4)和慢性胰腺炎组织(40.8±7.93)中微血管密度明显高于对照组(9.85±2.86)(P＜0.01).24例胰腺癌组织中p21ras阳性占15例,24例胰腺炎组织中p21ras阳性占11例,两者无显著差异,(x2=1.34,P＞0.05).p21ras阳性胰腺癌组织MVD(52.1±8.3)明显高于p21ras阴性的胰腺癌(39.1±4.1)及慢性胰腺炎组织(35.99±3.36)(P＜0.01),p21ras阳性的慢性胰腺炎组织MVD亦高于p21ras阴性的慢性胰腺炎组织(P＜0.01).结论微血管生成与K-ras突变关系密切,可能是胰腺癌发生发展中的早期事件,在慢性胰腺炎、胰腺癌的演变中亦起了不可低估的作用.
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